Novel Cysteine-Centered Sulfur Metabolic Pathway in the ThermotolerantMethylotrophic Yeast Hansenula polymorpha

Collection with item attached
2014
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/482503.do
DOI
10.1371/journal.pone.0100725
Title
Novel Cysteine-Centered Sulfur Metabolic Pathway in the ThermotolerantMethylotrophic Yeast Hansenula polymorpha
Description
Funding provided by the National Research Foundation of Korea (NRF)grant No. NRF-2012-0001150, http://www.nrf.re.kr/nrf_eng_cms/ and theNational Research Foundation of Korea (NRF) grant No.NRF-2013M3A6A8073554, http://www.nrf.re.kr/nrf_eng_cms/. The funders hadno role in study design, data collection and analysis, decision topublish, or preparation of the manuscript.
abstract
In yeast and filamentous fungi, sulfide can be condensed either with O-acetylhomoserine to generate homocysteine, the precursor of methionine, or with O-acetylserine to directly generate cysteine. The resulting homocysteine and cysteine can be interconverted through transsulfuration pathway. Here, we systematically analyzed the sulfur metabolic pathway of the thermotolerant methylotrophic yeast Hansenula polymorpha, which has attracted much attention as an industrial yeast strain for various biotechnological applications. Quite interestingly, the detailed sulfur metabolic pathway of H. polymorpha, which was reconstructed based on combined analyses of the genome sequences and validation by systematic gene deletion experiments, revealed the absence of de novo synthesis of homocysteine from inorganic sulfur in this yeast. Thus, the direct biosynthesis of cysteine from sulfide is the only pathway of synthesizing sulfur amino acids from inorganic sulfur in H. polymorpha, despite the presence of both directions of transsulfuration pathway Moreover, only cysteine, but no other sulfur amino acid, was able to repress the expression of a subset of sulfur genes, suggesting its central and exclusive role in the control of H. polymorpha sulfur metabolism. S-35-Cys was more efficiently incorporated into intracellular sulfur compounds such as glutathione than S-35-Met in H. polymorpha, further supporting the cysteine-centered sulfur pathway. This is the first report on the novel features of H. polymorpha sulfur metabolic pathway, which are noticeably distinct from those of other yeast and filamentous fungal species.
provenance
Made available in Cube on 2018-09-28T14:21:39Z (GMT). No. of bitstreams: 0
language
English
author
Sohn, Min Jeong
Yoo, Su Jin
Oh, Doo-Byoung
Kwon, Ohsuk
Lee, Sang Yup
Sibirny, Andriy A.
Kang, Hyun Ah
orcid
Lee, Sang Yup/0000-0003-0599-3091; Oh, Doo-Byoung/0000-0002-4432-0941
accessioned
2018-09-28T14:21:39Z
available
2018-09-28T14:21:39Z
issued
2014
citation
PLOS ONE(9): 6
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/482503.do
Funder
미래창조과학부
Funding Program
선도연구센터지원
Project ID
1711011855
Jurisdiction
Rep.of Korea
Project Name
Center for Fungal Pathogenesis
rights
openAccess
type
article


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