Monocyte Chemoattractant Protein-1 Deficiency Attenuates OxidativeStress and Protects against Ovariectomy-Induced Chronic Inflammation inMice

Collection with item attached
2013
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/481984.do
DOI
10.1371/journal.pone.0072108
Title
Monocyte Chemoattractant Protein-1 Deficiency Attenuates OxidativeStress and Protects against Ovariectomy-Induced Chronic Inflammation inMice
Description
This work was supported by a grant from the Korea Healthcare TechnologyR&D Project, Ministry for Health, Welfare & Family Affairs (A111295).The funders had no role in study design, data collection and analysis,decision to publish, or preparation of the manuscript.
abstract
Background: Loss of ovarian function is highly associated with an elevated risk of metabolic disease. Monocyte chemoattractant protein-1 (MCP-1, C-C chemokine ligand 2) plays critical roles in the development of inflammation, but its role in ovariectomy (OVX)-induced metabolic disturbance has not been known.
Methodology and Principal Findings: We investigated the role of MCP-1 in OVX-induced metabolic perturbation using MCP-1-knockout mice. OVX increased fat mass, serum levels of MCP-1, macrophage-colony stimulating factor (M-CSF), and reactive oxygen species (ROS), whereas MCP-1 deficiency attenuated these. OVX-induced increases of visceral fat resulted in elevated levels of highly inflammatory CD11c-expressing cells as well as other immune cells in adipose tissue, whereas a lack of MCP-1 significantly reduced all of these levels. MCP-1 deficiency attenuated activation of phospholipase C gamma 2, transforming oncogene from Ak strain, and extracellular signal-regulated kinase as well as generation of ROS, which is required for up-regulating CD11c expression upon M-CSF stimulation in bone marrow-derived macrophages.
Conclusions/Significance: Our data suggested that MCP-1 plays a key role in developing metabolic perturbation caused by a loss of ovarian functions through elevating CD11c expression via ROS generation.
provenance
Made available in Cube on 2018-09-28T14:07:35Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Woon-Ki
Choi, Eun-Kyung
Sul, Ok-Joo
Park, Yeon-Kyung
Kim, Eun-Sook
Yu, Rina
Suh, Jae-Hee
Choi, Hye-Seon
accessioned
2018-09-28T14:07:35Z
available
2018-09-28T14:07:35Z
issued
2013
citation
PLOS ONE(8): 8
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/481984.do
Funder
보건복지부
Funding Program
질환극복기술개발
Project ID
1465013676
Jurisdiction
Rep.of Korea
Project Name
Role of heme oxygenase-1 in bone remodeling
rights
openAccess
type
article


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