Modulation of experimental atopic dermatitis by topical application ofGami-Cheongyeul-Sodok-Eum

Collection with item attached
2013
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/481898.do
DOI
10.1186/1472-6882-13-312
Title
Modulation of experimental atopic dermatitis by topical application ofGami-Cheongyeul-Sodok-Eum
Description
This study was supported by grants from the Korean Health Technology R&DProject, Ministry of Health & Welfare, Republic of Korea (A100442 andB090072), Research program for Agricultural Science & TechnologyDevelopment (Project No: PJ907153), National Academy of AgriculturalScience, Rural Development Administration, Republic of Korea, and by theKorea Food Research Institute.
abstract
Background: Gami-Cheongyeul-Sodok-Eum (GCSE), an herbal formula of traditional Korean medicine, comprises nine herb components. GCSE has various biological activities such as anti-inflammatory, anti-bacterial and anti-viral activities. However, it is still unclear whether GCSE has any immunomodulatory effect on atopic dermatitis (AD).
Methods: GCSE was treated to primary B cells and CD4+ T cells isolated from atopic mice to compare its inhibitory effects on IgE secretion and cytokine expression. Experimental AD was established by alternative treatment of 2, 4-dinitrochlorobenzene (DNCB) and house dust mite extract to the ears of BALB/c mice. GCSE was topically applied to ears of atopic mice every day for 3 weeks. AD progression was analyzed by measuring ear thickness, serum IgE level, histological examination of ear tissue by H&E staining and cytokine profile of CD4+ T cells and CD19+ B cells by real time PCR and ELISA.
Results: Treatment of GCSE significantly reduced IgE production and expression of AD associated pathogenic cytokines such as IL-4, IL-5, IL-10, IL-13, IL-17, TNF-alpha, and IFN-gamma by lymphocytes isolated from AD-induced mice. Topical application of GCSE on the ears of AD-induced mice significantly reduced ear thickness, clinical score and lymphocytes infiltration to ears as compared to control group. GCSE treatment also reduced serum IgE level and the levels of major pathogenic cytokines such as IL-4, IL-5, IL-10, IL-13 and IL-17. In addition, GCSE treatment significantly increased Foxp3 expression level.
Conclusions: The protective effect of GCSE in experimental AD is mediated by inhibition of IgE production, by reduction in the levels of pathogenic cytokines and by induction of Foxp3, all of which are suggesting the beneficial effect of GCSE on modulating atopic dermatitis.
provenance
Made available in Cube on 2018-09-28T14:05:17Z (GMT). No. of bitstreams: 0
language
English
author
Hwang, Ji Sun
Kim, Jung-Eun
Yu, Young-Beob
Im, Sin-Hyeog
orcid
Im, Sin-Hyeog/0000-0002-3173-1856
accessioned
2018-09-28T14:05:17Z
available
2018-09-28T14:05:17Z
issued
2013
citation
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(13)
issn
1472-6882
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/481898.do
Funder
보건복지부
Funding Program
보건의료기술연구개발
Project ID
1465010009
Jurisdiction
Rep.of Korea
Project Name
Development of therapeutic modality for atopic dermatitis through regulation of IL-31 expression in CD4 T cells
rights
openAccess
subject
Atopic dermatitis
Herbal medicine
GCSE (Gami-Cheongyeul-Sodok-Eum)
Th1 & Th2 cells
B cells
IgE and Cytokines
type
article


Files in This Item

There are no attached files.