Large-scale transcriptome sequencing and gene analyses in thecrab-eating macaque (Macaca fascicularis) for biomedical research

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/481178.do
DOI
10.1186/1471-2164-13-163
Title
Large-scale transcriptome sequencing and gene analyses in thecrab-eating macaque (Macaca fascicularis) for biomedical research
Description
This research was supported by a grant from the KRIBB ResearchInitiative Program (KGM4241231).
abstract
Background: As a human replacement, the crab-eating macaque (Macaca fascicularis) is an invaluable non-human primate model for biomedical research, but the lack of genetic information on this primate has represented a significant obstacle for its broader use.
Results: Here, we sequenced the transcriptome of 16 tissues originated from two individuals of crab-eating macaque (male and female), and identified genes to resolve the main obstacles for understanding the biological response of the crab-eating macaque. From 4 million reads with 1.4 billion base sequences, 31,786 isotigs containing genes similar to those of humans, 12,672 novel isotigs, and 348,160 singletons were identified using the GS FLX sequencing method. Approximately 86% of human genes were represented among the genes sequenced in this study. Additionally, 175 tissue-specific transcripts were identified, 81 of which were experimentally validated. In total, 4,314 alternative splicing (AS) events were identified and analyzed. Intriguingly, 10.4% of AS events were associated with transposable element (TE) insertions. Finally, investigation of TE exonization events and evolutionary analysis were conducted, revealing interesting phenomena of human-specific amplified trends in TE exonization events.
Conclusions: This report represents the first large-scale transcriptome sequencing and genetic analyses of M. fascicularis and could contribute to its utility for biomedical research and basic biology.
provenance
Made available in Cube on 2018-09-28T13:45:50Z (GMT). No. of bitstreams: 0
language
English
author
Huh, Jae-Won
Kim, Young-Hyun
Park, Sang-Je
Kim, Dae-Soo
Lee, Sang-Rae
Kim, Kyoung-Min
Jung, Kang-Jin
Kim, Ji-Su
Song, Bong-Seok
Sim, Bo-Woong
Kim, Sun-Uk
Kim, Sang-Hyun
Chang, Kyu-Tae
orcid
Lee, Sang-Rae/0000-0001-8400-5973
accessioned
2018-09-28T13:45:50Z
available
2018-09-28T13:45:50Z
issued
2012
citation
BMC GENOMICS(13)
issn
1471-2164
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/481178.do
Funder
교육과학기술부
Funding Program
한국생명공학연구원연구운영비지원
Project ID
1345195582
Jurisdiction
Rep.of Korea
Project Name
National Primate Research Center Project
rights
openAccess
type
article


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