Increased expression of stathmin and elongation factor 1 alpha inprecancerous nodules with telomere dysfunction in hepatitis B viralcirrhotic patients

Collection with item attached
2014
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/480625.do
DOI
10.1186/1479-5876-12-154
Title
Increased expression of stathmin and elongation factor 1 alpha inprecancerous nodules with telomere dysfunction in hepatitis B viralcirrhotic patients
Description
This study was supported by a grant from the Korea Health Technology R&DProject through the Korea Health Industry Development Institute (KHIDI),funded by the Ministry of Health & Welfare, Republic of Korea (grantnumber: HI12C0555).
abstract
Background: Telomere dysfunction is important in carcinogenesis, and recently, stathmin and elongation factor (EF1 alpha) were reported to be up-regulated in telomere dysfunctional mice.
Methods: In the present study, the expression levels of stathmin and EF1 alpha in relation to telomere length, telomere dysfunction-induced foci (TIF), gamma-H2AX, and p21(WAF1/CIP1) expression were assessed in specimens of hepatitis B virus (HBV)-related multistep hepatocarcinogenesis, including 13 liver cirrhosis specimens, 14 low-grade dysplastic nodules (DN), 17 high-grade DNs, and 14 hepatocellular carcinomas (HCC). Five normal liver specimens were used as controls. TIF were analyzed by telomere fluorescent in situ hybridization (FISH) combined with immunostaining, while the protein expressions of stathmin, EF1 alpha, gamma-H2AX, and p21(WAF1/CIP1) were detected by immunohistochemistry.
Result: The expressions of stathmin and EF1 alpha gradually increased as multistep hepatocarcinogenesis progressed, showing the highest levels in HCC. Stathmin mRNA levels were higher in high-grade DNs than normal liver and liver cirrhosis, whereas EF1 alpha mRNA expression did not show such a difference. The protein expressions of stathmin and EF1 alpha were found in DNs of precancerous lesions, whereas they were absent or present at very low levels in normal liver and liver cirrhosis. Stathmin histoscores were higher in high-grade DNs and low-grade DNs than in normal liver (all, P < 0.05). EF1 alpha histoscores were higher in high-grade DNs than in normal liver and liver cirrhosis (all, P < 0.05). Stathmin mRNA levels and histoscores, as well as EF1 alpha histoscores (but not mRNA levels), were positively correlated with telomere shortening and gamma-H2AX labeling index (all, P < 0.05). EF1 alpha histoscores were also positively correlated with TIF (P < 0.001). Significantly greater inactivation of p21(WAF1/CIP1) was observed in low-grade DNs, high-grade DNs, and HCC, compared to liver cirrhos
provenance
Made available in Cube on 2018-09-28T13:30:52Z (GMT). No. of bitstreams: 0
language
English
author
Ahn, Ei Yong
Yoo, Jeong Eun
Rhee, Hyungjin
Kim, Myung Soo
Choi, Junjeong
Ko, Jung Eun
Lee, Jee San
Park, Young Nyun
orcid
Rhee, Hyungjin/0000-0001-7759-4458
accessioned
2018-09-28T13:30:52Z
available
2018-09-28T13:30:52Z
issued
2014
citation
JOURNAL OF TRANSLATIONAL MEDICINE(12)
issn
1479-5876
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/480625.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345228981
Jurisdiction
Rep.of Korea
Project Name
Project of Yonsei Medical Science
rights
openAccess
subject
Stathmin
Elongation factor 1 alpha
Telomere dysfunction
Hepatocarcinogenesis
Dysplastic nodule
Hepatocellular carcinoma
Hepatitis B virus
type
article


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