In vivo 3D measurement of moxifloxacin and gatifloxacin distributions inthe mouse cornea using multiphoton microscopy

아이템이 속한 컬렉션
2016
아이템 상세 URI
http://open-repository.kisti.re.kr/cube/handle/open_repository/480569.do
DOI
10.1038/srep25339
Title
In vivo 3D measurement of moxifloxacin and gatifloxacin distributions inthe mouse cornea using multiphoton microscopy
Description
Supported in part by Engineering Research Center (No. 2011-0030075), Bioand Medical Technology Development Program (NO. 2011-0019633) of theNational Research Foundation (NRF), Korea, NRF grant(NRF-2014R1A2A1A12067510), National R&D Program for Cancer Control (No.1320220) by National Cancer Center Korea, and Industrial TechnologyInnovation Program (No. 10048358) funded by the Ministry of Trade,Industry & Energy (MI, Korea), and Asan Institute for Life Science grant(No. 2014-7202), Asan Medical Center, Korea.
abstract
Moxifloxacin and gatifloxacin are fourth-generation fluoroquinolone antibiotics used in the clinic to prevent or treat ocular infections. Their pharmacokinetics in the cornea is usually measured from extracted ocular fluids or tissues, and in vivo direct measurement is difficult. In this study multiphoton microscopy (MPM), which is a 3D optical microscopic technique based on multiphoton fluorescence, was applied to the measurement of moxifloxacin and gatifloxacin distribution in the cornea. Intrinsic multiphoton fluorescence properties of moxifloxacin and gatifloxacin were characterized, and their distributions in mouse cornea in vivo were measured by 3D MPM imaging. Both moxifloxacin and gatifloxacin had similar multiphoton spectra, while moxifloxacin had stronger fluorescence than gatifloxacin. MPM imaging of mouse cornea in vivo showed (1) moxifloxacin had good penetration through the superficial corneal epithelium, while gatifloxacin had relatively poor penetration, (2) both ophthalmic solutions had high intracellular distribution. In vivo MPM results were consistent with previous studies. This study demonstrates the feasibility of MPM as a method for in vivo direct measurement of moxifloxacin and gatifloxacin in the cornea.
provenance
Made available in Cube on 2018-09-28T13:29:21Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Seunghun
Lee, Jun Ho
Park, Jin Hyoung
Yoon, Yeoreum
Chung, Wan Kyun
Tchah, Hungwon
Kim, Myoung Joon
Kim, Ki Hean
accessioned
2018-09-28T13:29:21Z
available
2018-09-28T13:29:21Z
issued
2016
citation
SCIENTIFIC REPORTS(6)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/480569.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345258214
Jurisdiction
Rep.of Korea
Project Name
Mechanical Engineering for Creative Emerging Technologies
rights
openAccess
type
article


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