Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis throughRaising Skin Level of ROS

Collection with item attached
2014
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/480186.do
DOI
10.1371/journal.pone.0109297
Title
Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis throughRaising Skin Level of ROS
Description
This research was supported by Basic Science Research Program throughthe National Research Foundation of Korea (NRF) funded by the Ministryof Education, Science and Technology (2013R1A1A2008077), RP-Grant 2010of Ewha Womans University and Institute for Basic Science (IBS). Thefunders had no role in study design, data collection and analysis,decision to publish, or preparation of the manuscript.
abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease resulting from excessive stimulation of immune cells. Traditionally, reactive oxygen species (ROS) have been implicated in the progression of inflammatory diseases, but several opposing observations suggest the protective role of ROS in inflammatory disease. Recently, we demonstrated ROS prevented imiquimod-induced psoriatic dermatitis through enhancing regulatory T cell function. Thus, we hypothesized AD might also be attenuated in elevated levels of ROS through tissue hyperoxygenation, such as by hyperbaric oxygen therapy (HBOT) or applying an oxygen-carrying chemical, perfluorodecalin (PFD). Elevated levels of ROS in the skin have been demonstrated directly by staining with dihydroethidum as well as indirectly by immunohistochemistry (IHC) for indoleamine 2,3-dioxygenase (IDO). A murine model of AD was developed by repeated application of a chemical irritant (1% 2,4-dinitrochlorobenzene) and house dust mite (Dermatophagoide farinae) extract on one ear of BALB/c mice. The results showed treatment with HBOT or PFD significantly attenuated AD, comparably with 0.1% prednicarbate without any signs of side effects, such as telangiectasia. The expressions of interleukin-17A and interferon-gamma were also decreased in the AD lesions by treatment with HBOT or PFD. Enhanced expression of IDO and reduced level of hypoxia-inducible factor-1 alpha, in association with increased frequency of FoxP3(+) regulatory T cells in the AD lesions, might be involved in the underlying mechanism of oxygen therapy. Taken together, it was suggested that tissue hyperoxygenation, by HBOT or treatment with PFD, might attenuate AD through enhancing skin ROS level.
provenance
Made available in Cube on 2018-09-28T13:19:13Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Hyung-Ran
Kim, Jung-Hwan
Choi, Eun-Jeong
Lee, Yeo Kyong
Kie, Jeong-Hae
Jang, Myoung Ho
Seoh, Ju-Young
orcid
Jang, Myoung Ho/0000-0001-8909-4725; Kie, Jeong Hae/0000-0003-1782-5650
accessioned
2018-09-28T13:19:13Z
available
2018-09-28T13:19:13Z
issued
2014
citation
PLOS ONE(9): 10
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/480186.do
Funder
미래창조과학부
Funding Program
기초과학연구원연구운영비지원
Project ID
1711017879
Jurisdiction
Rep.of Korea
Project Name
Immunology and Microbiology
rights
openAccess
type
article


Files in This Item

There are no attached files.