Histamine-HisCl1 Receptor Axis Regulates Wake-Promoting Signals inDrosophila melanogaster

Collection with item attached
2013
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/480027.do
DOI
10.1371/journal.pone.0068269
Title
Histamine-HisCl1 Receptor Axis Regulates Wake-Promoting Signals inDrosophila melanogaster
Description
This work was supported by grants from the Brain Research Center of the21st Century Frontier Research Program through the National ResearchFoundation of Korea funded by the Ministry of Education, Science andTechnology (MEST), the Republic of Korea, and from the National ResearchFoundation of Korea grant funded by MEST (No. 20110015442). The fundershad no role in study design, data collection and analysis, decision topublish, or preparation of the manuscript.
abstract
Histamine and its two receptors, histamine-gated chloride channel subunit 1 (HisCl1) and ora transientless (Ort), are known to control photoreception and temperature sensing in Drosophila. However, histamine signaling in the context of neural circuitry for sleep-wake behaviors has not yet been examined in detail. Here, we obtained mutant flies with compromised or enhanced histamine signaling and tested their baseline sleep. Hypomorphic mutations in histidine decarboxylase (HDC), an enzyme catalyzing the conversion from histidine to histamine, caused an increase in sleep duration. Interestingly, hisCl1 mutants but not ort mutants showed long-sleep phenotypes similar to those in hdc mutants. Increased sleep duration in hisCl1 mutants was rescued by overexpressing hisCl1 in circadian pacemaker neurons expressing a neuropeptide pigment dispersing factor (PDF). Consistently, RNA interference (RNAi)-mediated depletion of hisCl1 in PDF neurons was sufficient to mimic hisCl1 mutant phenotypes, suggesting that PDF neurons are crucial for sleep regulation by the histamine-HisCl1 signaling. Finally, either hisCl1 mutation or genetic ablation of PDF neurons dampened wake-promoting effects of elevated histamine signaling via direct histamine administration. Taken together, these data clearly demonstrate that the histamine-HisCl1 receptor axis can activate and maintain the wake state in Drosophila and that wake-activating signals may travel via the PDF neurons.
provenance
Made available in Cube on 2018-09-28T13:15:01Z (GMT). No. of bitstreams: 0
language
English
author
Oh, Yangkyun
Jang, Donghoon
Sonn, Jun Young
Choe, Joonho
accessioned
2018-09-28T13:15:01Z
available
2018-09-28T13:15:01Z
issued
2013
citation
PLOS ONE(8): 7
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/480027.do
Funder
미래창조과학부
Funding Program
중견연구자지원
Project ID
1345201969
Jurisdiction
Rep.of Korea
Project Name
Regulation of circadian rhythm and sleep mechanisms in Drosophila
rights
openAccess
type
article


Files in This Item

There are no attached files.