H-1 NMR-based metabolite profiling of diet-induced obesity in a mousemode

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/479722.do
DOI
10.5483/BMBRep.2012.45.7.248
Title
H-1 NMR-based metabolite profiling of diet-induced obesity in a mousemode
Description
This work was supported by a National Research Foundation grant (No.2011-0020603 and 2011-0027837 to Seong JK and No. 2011-0028272 and No.2010-0019394 to Hwang GS) funded by the Ministry of Education, Scienceand Technology of Korea (MEST) and a grant from the Korea Basic ScienceInstitute (T31409).
abstract
High-fat diets (HFD) and high-carbohydrate diets (HCD)-induced obesity through different pathways, but the metabolic differences between these diets are not fully understood. Therefore, we applied proton nuclear magnetic resonance (H-1 NMR)-based metabolomics to compare the metabolic patterns between C57BL/6 mice fed HCD and those fed HID. Principal component analysis derived from H-1 NMR spectra of urine showed a clear separation between the HCD and HFD groups. Based on the changes in urinary metabolites, the slow rate of weight gain in mice fed the HCD related to activation of the tricarboxylic acid cycle (resulting in increased levels of citrate and succinate in HCD mice), while the HFD affected nicotinamide metabolism (increased levels of 1-methylnicotineamide, nicotinamide-N-oxide in HID mice), which leads to systemic oxidative stress. In addition, perturbation of gut microflora metabolism was also related to different metabolic patterns of those two diets. These findings demonstrate that H-1 NMR-based metabolomics can identify diet-dependent perturbations in biological pathways. [BMB Reports 2012; 45(7): 419-424]
provenance
Made available in Cube on 2018-09-28T13:07:02Z (GMT). No. of bitstreams: 0
language
English
author
Jung, Jee-youn
Kim, Il Yong
Kim, Yo Na
Kim, Jin-sup
Shin, Jae Hoon
Jang, Zi-hey
Lee, Ho-Sub
Hwang, Geum-Sook
Seong, Je Kyung
accessioned
2018-09-28T13:07:02Z
available
2018-09-28T13:07:02Z
issued
2012
citation
BMB REPORTS(45): 7
issn
1976-6696
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/479722.do
Funder
교육과학기술부
Funding Program
한국기초과학지원연구원연구운영비지원
Project ID
1345194973
Jurisdiction
Rep.of Korea
Project Name
Development of an element equipments
rights
openAccess
subject
Etabolomics
High-fat diet
High-carbohydrate diet
Metaboliteprofiling
H-1 NMR
type
article


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