Growth of Ovarian Primary Follicles Retrieved from Neonates of DifferentAges and Derivation of Mature Oocytes Following In vitro-Culture

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/479697.do
DOI
10.5713/ajas.2010.10249
Title
Growth of Ovarian Primary Follicles Retrieved from Neonates of DifferentAges and Derivation of Mature Oocytes Following In vitro-Culture
Description
The submitted materials were partially presented in platformpresentation session of 2007 annual meeting of society for the study ofreproduction held in San Antonio, USA. This research was supported by agrant (SC-5160) from the Stem Cell Research Center of the 21st CenturyFrontier Research program funded by the Ministry of Education, Scienceand Technology (MEST) of the Republic of Korea. The authors alsoacknowledge a World Class University (WCU) program (R-31-10056), theNational Research Foundation of Korea funded by the MEST.
abstract
This study was conducted to improve the yield of mature oocytes from in vitro-culture of ovarian primary follicles by optimizing follicle retrieval from neonatal mice of different ages. Primary follicles of 75 to 99 pm in diameter were collected daily from 7- to 14-day-old neonatal mice, and subsequently cultured in alpha-MEM medium. Number of primary follicles isolated, growth of the follicle during in vitro-culture and maturation of intrafollicular oocytes were monitored. Overall, mean number of preantral follicles per animal was improved from 10.7 to 88.7 as the age of follicle donors was increased from 7 to 14-day-old. Number of primary follicles was increased gradually up to 11-day-old (35.7 follicle per an animal), then reduced to 29 in 14-day-old (p = 0.0013). More follicles retrieved from 10-day-old or 11-day-old females maintained their morphological normality at the end of primary culture than the follicles retrieved from 9-day-old. Of those cultured, primary follicles retrieved from 11-day-old mice yielded largest larger number of early secondary follicles than the follicles retrieved from in the other ages (39 vs. 13 to 29%). More than 3.3-times increase (0.86 to 2.86; p<0.05) in an average number of mature oocytes per animal was observed in the group of 11-day-old, compared with 9-day-old. However, no difference was found in the percentage of primary follicles developing into the pseudoantral stage (21 to 30%; p = 0.5222) and in the percentage of oocytes mucified (32 to 39%; p = 0.5792). In conclusion, a positive correlation between retrieval time and follicle growth was detected, which influences the efficiency to derive mature oocytes by follicle culture.
provenance
Made available in Cube on 2018-09-28T13:06:23Z (GMT). No. of bitstreams: 0
language
English
author
Choi, J. H.
Yoo, C. R.
Ahn, J. Y.
Park, J. H.
Lim, J. M.
accessioned
2018-09-28T13:06:23Z
available
2018-09-28T13:06:23Z
issued
2012
citation
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES(25): 5
issn
1011-2367
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/479697.do
Funder
교육과학기술부
Funding Program
세계수준의연구중심대학육성_일반(0.5)
Project ID
1345196329
Jurisdiction
Rep.of Korea
Project Name
Multidisciplinary research network in biomodel development and functional modulation
rights
openAccess
subject
Neonatal Mouse
Ovary
Primary Follicle
In vitro Culture
OocyteMaturation
type
article


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