Growth Inhibitory Effect of Palatine Tonsil-derived Mesenchymal StemCells on Head and Neck Squamous Cell Carcinoma Cells

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/479689.do
DOI
10.3342/ceo.2012.5.2.86
Title
Growth Inhibitory Effect of Palatine Tonsil-derived Mesenchymal StemCells on Head and Neck Squamous Cell Carcinoma Cells
Description
This study was supported by Medical Research Institute and Busan CancerCenter Grant (2010-27), Pusan National University Hospital.
abstract
Objectives. Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. However, the effect of human MSCs on the growth of human tumors is not well understood. The purpose of this study is to confirm the growth effect of palatine tonsil-derived MSCs (TD-MSCs) on head and neck squarnous cell carcinoma (HNSCC) cell lines and to elucidate the mechanism of their action.
Methods. TD-MSCs were isolated from patient with chronic tonsillitis and tonsillar hypertrophy. Two human HNSCC cell lines (PNUH-12 and SNU-899) were studied and cocultured with isolated palatine tonsil-derived MSC. The growth inhibitory effect of MSCs on HNSCC cell lines was tested through methylthiazolyldiphenyl-tetrazolium (MTT) assay. The apoptosis induction effect of MSCs on cell lines was assessed with flow cytometry and reverse transcriptase (RT)-PCR.
Results. Palatine tonsil-derived MSCs exhibited a growth inhibitory effect on both cell lines. Cell cycle analysis showed an accumulation of tumor cells predominantly in G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. Apoptosis of tumor cells increased significantly as concentration of cocultured TD-MSCs increased. Additionally, mRNA expression of caspase 3 was upregulated with increased concentration of TD-MSCs.
Conclusion. TD-MSCs have a potential growth inhibitory effect on HNSCC cell lines in vitro by inducing apoptotic cell death and G1 phase arrest of cell lines.
provenance
Made available in Cube on 2018-09-28T13:06:10Z (GMT). No. of bitstreams: 0
language
English
author
Lim, Yun-Sung
Lee, Jin-Choon
Lee, Yoon Se
Lee, Byung-Joo
Wang, Soo-Geun
accessioned
2018-09-28T13:06:10Z
available
2018-09-28T13:06:10Z
issued
2012
citation
CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY(5): 2
issn
1976-8710
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/479689.do
Funder
교육과학기술부
Funding Program
2단계연구중심대학육성(0.5)
Project ID
1345196629
Jurisdiction
Rep.of Korea
Project Name
Pusan National University BK21 Medical science Education Center
rights
openAccess
subject
Apoptosis
Mesenchymal stem cells
Palatine tonsil
Squamous cellcarcinoma
type
article


Files in This Item

There are no attached files.