Functional and Molecular Changes of the Bladder in Rats with CrushingInjury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Roleof RhoA/Rho Kinase Pathway

Collection with item attached
2013
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/479254.do
DOI
10.3390/ijms140917511
Title
Functional and Molecular Changes of the Bladder in Rats with CrushingInjury of Nerve Bundles from Major Pelvic Ganglion to the Bladder: Roleof RhoA/Rho Kinase Pathway
Description
This study was supported by the Korean Health Technology R&D Project,Ministry of Health & Welfare, Republic of Korea (A111055).
abstract
Voiding dysfunction is a common complication after radical pelvic surgery. To reduce this complication, nerve-sparing radical pelvic surgery was introduced. However, several patients experienced voiding difficulty despite nerve-sparing radical pelvic surgery. Thus, we investigated the functional and molecular changes of the bladder in rats, which demonstrated voiding dysfunction induced by nerve damage during nerve-sparing radical pelvic surgery. Male rats were used and assigned to normal, sham-operated, and bilateral crushing nerve bundles from major pelvic ganglion (MPG) to bladder group. After one, two, and four-week crushing injury, significantly decreased contractile response and increased connective tissue of the detrusor were observed and these results were reliable findings with voiding difficulty following nerve-sparing radical pelvic surgery. After crushing injury, significantly increased M2 muscarinic receptor expression was observed and this might be regarded as the compensatory response. However, M3 muscarinic receptor expression was not significantly changed. The expression of RhoA, ROCK-, and ROCK- was significantly increased after one, two, and four-week crushing injury. From these results, the down-regulation of RhoA/Rho kinase pathway might lead to the decreased bladder contractility after crushing injury of nerve bundles from MPG to the bladder despite of the compensated up-regulation of M2 muscarinic receptor.
provenance
Made available in Cube on 2018-09-28T12:54:42Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Su Jin
Lee, Dong Sup
Bae, Woong Jin
Kim, Seol
Hong, Sung Hoo
Lee, Ji Youl
Hwang, Tae-Kon
Kim, Sae Woong
accessioned
2018-09-28T12:54:42Z
available
2018-09-28T12:54:42Z
issued
2013
citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(14): 9
issn
1422-0067
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/479254.do
Funder
보건복지부
Funding Program
의료기기기술개발
Project ID
1465012703
Jurisdiction
Rep.of Korea
Project Name
Development of status monitoring and therapeutic system for urologic diseases
rights
openAccess
subject
urinary bladder
receptor
muscarinic
Rho-associated kinase
autonomicganglia
injury
nerve crush
rat
type
article


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