Fabrication of Photofunctional Nanoporous Membrane and ItsPhotoinactivation Effect of Vesicular Stomatitis Virus

Collection with item attached
2011
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/478880.do
DOI
10.1155/2012/454507
Title
Fabrication of Photofunctional Nanoporous Membrane and ItsPhotoinactivation Effect of Vesicular Stomatitis Virus
Description
This study was supported by a grant of the Korea Healthcare TechnologyR&D Project, Ministry of Health & Family Affairs, Republic of Korea.(grant no. A085136). The authors are grateful to Prof. H. K. Kim for thegenerous support of PtCP and Dr. S. I. Oh for the help in thepreparation of NAM.
abstract
Fabrication and photophysical study of photofunctional nanoporous alumina membrane (PNAM) were performed, and its application of photodynamic antimicrobial chemotherapy (PACT) was investigated. Nanoporous alumina membrane (NAM) was fabricated by two-step aluminium anodic oxidation process. Surface of the fabricated NAM was modified with organo-silane agent to induce covalent bonding between NAM and a photosensitizer (PtCP: [5,10,15-triphenyl-20-(4-methoxycarbonylphenyl)porphyrin] platinum). PtCP was covalently bonded to the surface of the modified NAM by nucleophilic acyl substitution reaction process. The morphology and the photophysical properties of the fabricated PNAM were confirmed with field emission scanning electron microscope (FE-SEM), steady-state spectroscopies, and nanosecond laser-induced time-resolved spectroscopy. For the efficacy study of PNAM in PACT, an enveloped animal virus, vesicular stomatitis virus (VSV), was utilized as a target organism. Antiviral effect of the PNAM-PACT was measured by the extent of suppression of plaque-forming units (PFU) after the light irradiation. In the cultures inoculated with PACT-treated VSV, the suppression of PFU was prominent, which demonstrates that PNAM is a potential bio clean-up tool.
provenance
Made available in Cube on 2018-09-28T12:44:54Z (GMT). No. of bitstreams: 0
language
English
author
Wang, Kang-Kyun
Kim, Bong-Jin
Ko, Si-Hwan
Choi, Dong Hoon
Kim, Yong-Rok
orcid
Wang, Kang-kyun/0000-0001-5116-8151
accessioned
2018-09-28T12:44:54Z
available
2018-09-28T12:44:54Z
issued
2011
citation
JOURNAL OF NANOMATERIALS
issn
1687-4110
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/478880.do
Funder
교육과학기술부
Funding Program
2단계연구중심대학육성
Project ID
1345165040
Jurisdiction
Rep.of Korea
Project Name
Institute for NanoBio Molecular Assemblies
rights
openAccess
type
article


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