Co-culture of Melanocytes with Adipose-derived Stem Cells as a PotentialSubstitute for Co-culture with Keratinocytes

Collection with item attached
2011
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/476144.do
DOI
10.2340/00015555-1174
Title
Co-culture of Melanocytes with Adipose-derived Stem Cells as a PotentialSubstitute for Co-culture with Keratinocytes
Description
This study was supported by a grant from the Korea Healthcare TechnologyR&D Project (A080980), Ministry of Health Welfare and Family Affairs,Republic of Korea.
abstract
Cell-to-cell interactions between melanocytes and keratinocytes increase the proliferation and migration of melanocytes. In fact, mixed keratinocyte and melanocyte cultures have been used for autologous cell transplantation for treatment of vitiligo. However, this may require taking an amount of skin tissue large enough to leave scars. In this study, the in vitro effect of adipose-derived stem cells (ADSCs) on proliferation, differentiation and migration of melanocytes was compared with that of keratinocytes using immunohistochemistry and a Boyden chamber migration assay. The proliferation and migration of melanocytes was significantly stimulated by co-culture with ADSCs compared with melanocyte monocultures, although the effect of ADSCs was less powerful than that of keratinocytes. This may be related to increases in stem cell factor and basic fibroblast growth factor, growth factors for melanocytes, produced by the ADSCs. The ratios of melanocytes stained with antibodies against Trp-2, E-cadherin and N-cadherin were significantly increased by co-culturing with ADSCs compared with co-culturing with keratinocytes as well as melanocyte monocultures. The proportion of less-pigmented melanocytes was also increased and sustained for a longer duration in the presence of ADSCs. Our data show that co-culturing with ADSCs results in increased melanocyte proliferation and migration while reducing differentiation, and could provide a means to treat disorders such as vitiligo.
provenance
Made available in Cube on 2018-09-28T11:31:37Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Ji-Young
Do Park, Chan
Lee, Joon Ho
Lee, Chang-Hoon
Do, Byung-Rok
Lee, Ai-Young
accessioned
2018-09-28T11:31:37Z
available
2018-09-28T11:31:37Z
issued
2011
citation
ACTA DERMATO-VENEREOLOGICA(92): 1
issn
0001-5555
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/476144.do
Funder
보건복지가족부
Funding Program
보건의료기술연구개발
Project ID
1355059884
Jurisdiction
Rep.of Korea
Project Name
Study for effect of bee venom ingredients in skin melanocyte proliferation and migration
rights
openAccess
subject
adipose-derived stem cells
keratinocytes
co-culture
melanocytes
proliferation
differentiation
migration
type
article


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