Characterization of Molecular Mechanisms Controlling fabAB Transcriptionin Pseudomonas aeruginosa

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/475909.do
DOI
10.1371/journal.pone.0045646
Title
Characterization of Molecular Mechanisms Controlling fabAB Transcriptionin Pseudomonas aeruginosa
Description
This research was supported by Basic Science Research Program throughthe National Research Foundation of Korea (NRF) funded by the Ministryof Education, Science and Technology (2010-0004068). The funder had norole in study design, data collection and analysis, decision to publish,or preparation of the manuscript.
abstract
Background: The FabAB pathway is one of the unsaturated fatty acid (UFA) synthesis pathways for Pseudomonas aeruginosa. It was previously noted that this operon was upregulated in biofilms and repressed by exogenous UFAs. Deletion of a 30 nt fabA upstream sequence, which is conserved in P. aeruginosa, P. putida, and P. syringae, led to a significant decrease in fabA transcription, suggesting positive regulation by an unknown positive regulatory mechanism.
Methods/Principal Findings: Here, genetic and biochemical approaches were employed to identify a potential fabAB activator. Deletion of candidate genes such as PA1611 or PA1627 was performed to determine if any of these gene products act as a fabAB activator. However, none of these genes were involved in the regulation of fabAB transcription. Use of mariner-based random mutagenesis to screen for fabA activator(s) showed that several genes encoding unknown functions, rpoN and DesA may be involved in fabA regulation, but probably via indirect mechanisms. Biochemical attempts performed did fail to isolate an activator of fabAB operon.
Conclusion/Significance: The data suggest that fabA expression might not be regulated by protein-binding, but by a distinct mechanism such as a regulatory RNA-based mechanism.
provenance
Made available in Cube on 2018-09-28T11:25:25Z (GMT). No. of bitstreams: 0
language
English
author
Schweizer, Herbert P.
Choi, Kyoung-Hee
accessioned
2018-09-28T11:25:25Z
available
2018-09-28T11:25:25Z
issued
2012
citation
PLOS ONE(7): 10
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/475909.do
Funder
교육과학기술부
Funding Program
일반연구자지원
Project ID
1345153054
Jurisdiction
Rep.of Korea
Project Name
Establishment of a drug target via investigation of the mechanism governing the synthesis of unsaturated fatty acids in Pseudomonas aeruginosa
rights
openAccess
type
article


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