Characterization of LysB4, an endolysin from the Bacilluscereus-infecting bacteriophage B4

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/475907.do
DOI
10.1186/1471-2180-12-33
Title
Characterization of LysB4, an endolysin from the Bacilluscereus-infecting bacteriophage B4
Description
This work was supported by grants to S. Ryu from the AgricultureResearch Center program of the Ministry for Food, Agriculture, Forestryand Fisheries, Republic of Korea. BS, JL and HS were the recipients of agraduate fellowship provided by the MEST through the Brain Korea 21Project.
abstract
Background: Bacillus cereus is a foodborne pathogen that causes emetic or diarrheal types of food poisoning. The incidence of B. cereus food poisoning has been gradually increasing over the past few years, therefore, biocontrol agents effective against B. cereus need to be developed. Endolysins are phage-encoded bacterial peptidoglycan hydrolases and have received considerable attention as promising antibacterial agents.
Results: The endolysin from B. cereus phage B4, designated LysB4, was identified and characterized. In silico analysis revealed that this endolysin had the VanY domain at the N terminus as the catalytic domain, and the SH3_5 domain at the C terminus that appears to be the cell wall binding domain. Biochemical characterization of LysB4 enzymatic activity showed that it had optimal peptidoglycan hydrolase activity at pH 8.0-10.0 and 50 degrees C. The lytic activity was dependent on divalent metal ions, especially Zn2+. The antimicrobial spectrum was relatively broad because LysB4 lysed Gram-positive bacteria such as B. cereus, Bacillus subtilis and Listeria monocytogenes and some Gram-negative bacteria when treated with EDTA. LC-MS analysis of the cell wall cleavage products showed that LysB4 was an L-alanoyl-D-glutamate endopeptidase, making LysB4 the first characterized endopeptidase of this type to target B. cereus.
Conclusions: LysB4 is believed to be the first reported L-alanoyl-D-glutamate endopeptidase from B. cereus-infecting bacteriophages. The properties of LysB4 showed that this endolysin has strong lytic activity against a broad range of pathogenic bacteria, which makes LysB4 a good candidate as a biocontrol agent against B. cereus and other pathogenic bacteria.
provenance
Made available in Cube on 2018-09-28T11:25:22Z (GMT). No. of bitstreams: 0
language
English
author
Son, Bokyung
Yun, Jiae
Lim, Jeong-A
Shin, Hakdong
Heu, Sunggi
Ryu, Sangryeol
orcid
Shin, Hakdong/0000-0001-7615-9809
accessioned
2018-09-28T11:25:22Z
available
2018-09-28T11:25:22Z
issued
2012
citation
BMC MICROBIOLOGY(12)
issn
1471-2180
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/475907.do
Funder
농림수산식품부
Funding Program
융복합연구센터지원
Project ID
1545002742
Jurisdiction
Rep.of Korea
Project Name
Development of novel technologies for assurance of food-safety based on molecular control practices
rights
openAccess
type
article


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