Characterization of Insertional Variation of Porcine EndogenousRetroviruses in Six Different Pig Breeds

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/475904.do
DOI
10.5713/ajas.2012.12131
Title
Characterization of Insertional Variation of Porcine EndogenousRetroviruses in Six Different Pig Breeds
Description
This work was supported by a grant (No. 20070401034031) from BioGreen 21program, Rural Development Administration, Republic of Korea and by agrant (No. 20100023352) from the National Research Foundation of Korea,Republic of Korea. Also, authors give special thanks to Animal Genomicsand Bioinformatics Division in National Institute of Animal Science,RDA, for accessing RDA libraries.
abstract
Pigs may need to be exploited as xenotransplantation donors due to the shortage of human organs, tissues and cells. Porcine endogenous retroviruses (PERVs) are a significant obstacle to xenotransplantation because they can infect human cells in vitro and have the potential for transmission of unexpected pathogens to humans. In this research. 101 pigs, including four commercial breeds (23 Berkshire, 13 Duroc, 22 Landrace and 14 Yorkshire pigs), one native breed (19 Korean native pigs) and one miniature breed (10 NIH miniature pigs) were used to investigate insertional variations for 11 PERV loci (three PERV-A, six PERV-B and two PERV-C). Over 60% of the pigs harbored one PERV-A (907F8) integration and five PERV-B (B3-3G, B3-7G, 742H1, 1155D9 and 465D1) integrations. However, two PERV-A loci (A1-6C and 1347C1) and one PERV-B locus (B3-7F) were absent in Duroc pigs. Moreover, two PERV-C loci (C2-6C and C4-2G) only existed in Korean native pigs and NIH miniature pigs. The results suggest that PERV insertional variations differ among pig breeds as well as among individuals within a breed. Also, the results presented here can be used for the selection of animals that do not have specific PERV integration for xenotransplantation research.
provenance
Made available in Cube on 2018-09-28T11:25:17Z (GMT). No. of bitstreams: 0
language
English
author
Jung, W. Y.
Yu, S. L.
Seo, D. W.
Jung, K. C.
Cho, I. C.
Lim, H. T.
Jin, D. I.
Lee, J. H.
accessioned
2018-09-28T11:25:17Z
available
2018-09-28T11:25:17Z
issued
2012
citation
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES(25): 10
issn
1011-2367
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/475904.do
Funder
교육과학기술부
Funding Program
2단계연구중심대학육성(0.5)
Project ID
1345196895
Jurisdiction
Rep.of Korea
Project Name
Graduate Program for Creative Research in Animal Biotechnology
rights
openAccess
subject
Insertional Variation
Pig Breeds
Porcine Endogenous Retroviruses(PERVs)
Xenotransplantation
type
article


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