CEP90 Is Required for the Assembly and Centrosomal Accumulation ofCentriolar Satellites, Which Is Essential for Primary Cilia Formation

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/475797.do
DOI
10.1371/journal.pone.0048196
Title
CEP90 Is Required for the Assembly and Centrosomal Accumulation ofCentriolar Satellites, Which Is Essential for Primary Cilia Formation
Description
This study was supported by grants from the BioImaging Research Centerat GIST; the Basic Research Program (grant numbers 2010-0022423 and2012R1A2A201003512); the Science Research Center Program (grant number2011-0006425) of the Ministry of Education, Science and Technology; andthe second stage of the Brain Korea 21 Project. The funders had no rolein study design, data collection and analysis, decision to publish, orpreparation of the manuscript.
abstract
Centriolar satellites are PCM-1-positive granules surrounding centrosomes. Proposed functions of the centriolar satellites include protein targeting to the centrosome, as well as communication between the centrosome and surrounding cytoplasm. CEP90 is a centriolar satellite protein that is critical for spindle pole integrity in mitotic cells. In this study, we examined the biological functions of CEP90 in interphase cells. CEP90 physically interacts with PCM-1 at centriolar satellites, and this interaction is essential for centrosomal accumulation of the centriolar satellites and eventually for primary cilia formation. CEP90 is also required for BBS4 loading on centriolar satellites and its localization in primary cilia. Our results imply that the assembly and transport of centriolar satellites are critical steps for primary cilia formation and ciliary protein recruitment.
provenance
Made available in Cube on 2018-09-28T11:22:28Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Kyeongmi
Lee, Kwanwoo
Rhee, Kunsoo
accessioned
2018-09-28T11:22:28Z
available
2018-09-28T11:22:28Z
issued
2012
citation
PLOS ONE(7): 10
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/475797.do
Funder
교육과학기술부
Funding Program
2단계연구중심대학육성(0.5)
Project ID
1345196650
Jurisdiction
Rep.of Korea
Project Name
생명과학고급인력양성사업단
rights
openAccess
type
article


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