CD4(+)CD25(+) Regulatory T Cell Depletion Modulates Anxiety andDepression-Like Behaviors in Mice

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/475749.do
DOI
10.1371/journal.pone.0042054
Title
CD4(+)CD25(+) Regulatory T Cell Depletion Modulates Anxiety andDepression-Like Behaviors in Mice
Description
This work was supported by the Korea Science and Engineering Foundation(KOSEF) grant funded by the Korea government (MEST) ( No. 20090063466).The funders had no role in study design, data collection and analysis,decision to publish, or preparation of the manuscript.
abstract
Stress has been shown to suppress immune function and increase susceptibility to inflammatory disease and psychiatric disease. CD4(+)CD25(+) regulatory T (Treg) cells are prominent in immune regulation. This study was conducted to determine if anti-CD25 antibody (Ab) mediated depletion of Treg cells in mice susceptibility to stress-induced development of depression-like behaviors, as well as immunological and neurochemical activity. To accomplish this, an elevated plus-maze test (EPM), tail suspension test (TST), and forced swim test (FST) were used to examine depression-like behaviors upon chronic immobilization stress. Immune imbalance status was observed based on analysis of serum cytokines using a mouse cytometric bead array in conjunction with flow cytometry and changes in the levels of serotonin (5-HT) and dopamine (DA) in the brain were measured by high performance liquid chromatography (HPLC). The time spent in the open arms of the EPM decreased significantly and the immobility time in the FST increased significantly in the anti-CD25 Ab-treated group when compared with the non stressed wild-type group. In addition, interlukin-6 (IL-6), tumor necrosis factor-a (TNF-a), interlukin-2 (IL-2), interferon-gamma (IFN-gamma), interlukin-4 (IL-4) and interlukin-17A (IL-17A) concentrations were significantly upregulated in the stressed anti-CD25 Ab-treated group when compared with the non stressed wild-type group. Furthermore, the non stressed anti-CD25 Ab-treated group displayed decreased 5-HT levels within the hippocampus when compared with the non stressed wild-type group. These results suggest that CD4(+)CD25(+) Treg cell depletion modulated alterations in depressive behavior, cytokine and monoaminergic activity. Therefore, controlling CD4(+)CD25(+) Treg cell function during stress may be a potent therapeutic strategy for the treatment of depression-like symptoms.
provenance
Made available in Cube on 2018-09-28T11:21:12Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Soo-Jeong
Lee, Hyojung
Lee, Gihyun
Oh, Sei-Joong
Shin, Min-Kyu
Shim, Insop
Bae, Hyunsu
orcid
Bae, Hyunsu/0000-0002-0299-3582; Lee, Gihyun/0000-0001-6250-8852
accessioned
2018-09-28T11:21:12Z
available
2018-09-28T11:21:12Z
issued
2012
citation
PLOS ONE(7): 7
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/475749.do
Funder
교육과학기술부
Funding Program
2단계연구중심대학육성(0.5)
Project ID
1345196514
Jurisdiction
Rep.of Korea
Project Name
한의과학사업단
rights
openAccess
type
article


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