Cardiac Transcription Factor Nkx2.5 Is Downregulated under ExcessiveO-GlcNAcylation Condition

Collection with item attached
2012
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/475702.do
DOI
10.1371/journal.pone.0038053
Title
Cardiac Transcription Factor Nkx2.5 Is Downregulated under ExcessiveO-GlcNAcylation Condition
Description
This work was supported by National Research Foundation of Korea Grantfunded by the Korean Government (KRF-2006-311-C00399) and supported byBasic Science Research Program through the National Research Foundationof Korea (NRF) funded by the Ministry of Education, Science andTechnology (2011-0003657, 2011-0004322). The funders had no role instudy design, data collection and analysis, decision to publish, orpreparation of the manuscript.
abstract
Post-translational modification of proteins with O-linked N-acetylglucosamine (O-GlcNAc) is linked the development of diabetic cardiomyopathy. We investigated whether Nkx2.5 protein, a cardiac transcription factor, is regulated by O-GlcNAc. Recombinant Nkx2.5 (myc-Nkx2.5) proteins were reduced by treatment with the O-GlcNAcase inhibitors STZ and O-(2-acetamido-2-deoxy-D-glucopyroanosylidene)-amino-N-phenylcarbamate; PUGNAC) as well as the overexpression of recombinant O-GlcNAc transferase (OGT-flag). Co-immunoprecipitation analysis revealed that myc-Nkx2.5 and OGT-flag proteins interacted and myc-Nkx2.5 proteins were modified by O-GlcNAc. In addition, Nkx2.5 proteins were reduced in the heart tissue of streptozotocin (STZ)-induced diabetic mice and O-GlcNAc modification of Nkx2.5 protein increased in diabetic heart tissue compared with non-diabetic heart. Thus, excessive O-GlcNAcylation causes downregulation of Nkx2.5, which may be an underlying contributing factor for the development of diabetic cardiomyopathy.
provenance
Made available in Cube on 2018-09-28T11:19:57Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Hoe Suk
Woo, Ji Soo
Joo, Hyun Jung
Moon, Woo Kyung
accessioned
2018-09-28T11:19:57Z
available
2018-09-28T11:19:57Z
issued
2012
citation
PLOS ONE(7): 6
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/475702.do
Funder
교육과학기술부
Funding Program
중견연구자지원
Project ID
1345151818
Jurisdiction
Rep.of Korea
Project Name
In vivo tracking of cancer stem cell and mesenchymal stem cell interaction using multiple ferritin-fluorescent reporters
rights
openAccess
type
article


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