An Essential Role for TAGLN2 in Phagocytosis ofLipopolysaccharide-activated Macrophages

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474582.do
DOI
10.1038/s41598-017-09144-x
Title
An Essential Role for TAGLN2 in Phagocytosis ofLipopolysaccharide-activated Macrophages
Description
This work was supported by the Basic Science Program(2015R1A2A1A15052658) and the Creative Research Initiative Program(2015R1A3A2066253) through National Research Foundation (NRF) grantsfunded by the Ministry of Science, ICT & Future Planning (MSIP), andBasic Science Program (2013R1A6A3A04064259) through National ResearchFoundation (NRF) grants funded by the Ministry of Education (MOE),Korea, and the GIST Research Institute (GRI) in 2017.
abstract
Activated macrophages have a greater ability of phagocytosis against pathogens that is mediated by large-scale actin rearrangement. However, molecular machineries that conduct this task have not been fully identified. Here, we demonstrate an unanticipated role of TAGLN2, a 22-kDa actin-binding protein, in Toll-like receptor (TLR)-stimulated phagocytosis. TAGLN2 was greatly induced in macrophages in response to lipopolysaccharide (LPS), a ligand for TLR4, partly via the NF-kappa B pathway. TAGLN2-deficient macrophages (TAGLN2(-/-)) showed defective phagocytic functions of IgM- and IgG-coated sheep red blood cells as well as bacteria. Cell signaling pathways involved in actin rearrangement-PI3 kinase/AKT and Ras-ERK-were also down-regulated in LPS- stimulated TAGLN2-deficient macrophages. Moreover, TAGLN2(-/-) mice showed higher mortality after bacterial infection than wild-type littermates. Thus, our results revealed a novel function of TAGLN2 as a molecular armament required for host defense.
provenance
Made available in Cube on 2018-09-28T10:49:50Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Hye-Ran
Lee, Hyun-Su
Lee, Kyung-Sik
Jung, In Duk
Kwon, Min-Sung
Kim, Chang-Hyun
Kim, Seong-Min
Yoon, Myung-Han
Park, Yeong-Min
Lee, Sang-Myeong
Jun, Chang-Duk
accessioned
2018-09-28T10:49:50Z
available
2018-09-28T10:49:50Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474582.do
Funder
과학기술정보통신부
Funding Program
광주과학기술원연구운영비지원(0.5)
Project ID
1711061910
Jurisdiction
Rep.of Korea
Project Name
생명노화특성화연구
rights
openAccess
type
article


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