An endoparasitoid wasp influences host DNA methylation

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474562.do
DOI
10.1038/srep43287
Title
An endoparasitoid wasp influences host DNA methylation
Description
This study was supported by a general researcher program(2013R1A1A2A10058197) funded by National Research Foundation of Korea.
abstract
Parasitism by endoparasitoid wasps changes the expression of various host genes, and alters host immune and developmental processes. However, it is not clearly understood how parasitism changes host gene expression in a whole genome scale. This study focused on an epigenetic control of Cotesia plutellae, an endoparasitoid wasp, against its host, Plutella xylostella. Two DNA methyltransferases (DNMT-1 and DNMT-2) are encoded in the genome of P. xylostella. In addition, methyl-binding domain proteins (MBDs) and DNA demethylation factor, ten-eleven translation protein (TET) are encoded. DNA methylation of P. xylostella genomic DNA was confirmed by restriction digestion with Gla I specific to 5-methylcytosine. DNA methylation intensity in parasitized (P) larvae was decreased compared to that in nonparasitized (NP) larvae, especially at late parasitic stage, at which expression levels of both DNMT-1 and DNMT-2 were also decreased. DNA demethylation of P. xylostella was confirmed in both NP and P larvae by restriction digestion with PvuRts1I recognizing 5-hydroxymethyl cytosine. Parasitism also suppressed expression levels of TET and MBDs. Treatment of 5-aza-2'-deoxycytidine (AZA) reduced DNA methylation intensity of NP larvae, causing suppression of hemocyte-spreading behavior and delay of immature development. RNA interference of DNMT-1 or DNMT-2 mimicked the adverse effects of AZA.
provenance
Made available in Cube on 2018-09-28T10:49:18Z (GMT). No. of bitstreams: 0
language
English
author
Kumar, Sunil
Kim, Yonggyun
accessioned
2018-09-28T10:49:18Z
available
2018-09-28T10:49:18Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474562.do
Funder
교육부
Funding Program
이공학개인기초연구지원
Project ID
1345239255
Jurisdiction
Rep.of Korea
Project Name
Eicosanoid Cross-talk of Innate Immunity Signals in Insects
rights
openAccess
type
article


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