Alternative Splicing Profile and Sex-Preferential Gene Expression in theFemale and Male Pacific Abalone Haliotis discus hannai

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474471.do
DOI
10.3390/genes8030099
Title
Alternative Splicing Profile and Sex-Preferential Gene Expression in theFemale and Male Pacific Abalone Haliotis discus hannai
Description
This research was supported by the Golden Seed Project, Ministry ofAgriculture, Food and Rural Affairs (MAFRA), Ministry of Oceans andFisheries (MOF), Rural Development Administration (RDA), and KoreaForest Service (KFS), and by Basic Science Research Program through theNational Research Foundation of Korea (NRF) funded by the Ministry ofEducation (2015R1D1A1A01060673).
abstract
In order to characterize the female or male transcriptome of the Pacific abalone and further increase genomic resources, we sequenced the mRNA of full-length complementary DNA (cDNA) libraries derived from pooled tissues of female and male Haliotis discus hannai by employing the Iso-Seq protocol of the PacBio RSII platform. We successfully assembled whole full-length cDNA sequences and constructed a transcriptome database that included isoform information. After clustering, a total of 15,110 and 12,145 genes that coded for proteins were identified in female and male abalones, respectively. A total of 13,057 putative orthologs were retained from each transcriptome in abalones. Overall Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyzed in each database showed a similar composition between sexes. In addition, a total of 519 and 391 isoforms were genome-widely identified with at least two isoforms from female and male transcriptome databases. We found that the number of isoforms and their alternatively spliced patterns are variable and sex-dependent. This information represents the first significant contribution to sex-preferential genomic resources of the Pacific abalone. The availability of whole female and male transcriptome database and their isoform information will be useful to improve our understanding of molecular responses and also for the analysis of population dynamics in the Pacific abalone.
provenance
Made available in Cube on 2018-09-28T10:46:56Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Mi Ae
Rhee, Jae-Sung
Kim, Tae Ha
Lee, Jung Sick
Choi, Ah-Young
Choi, Beom-Soon
Choi, Ik-Young
Sohn, Young Chang
accessioned
2018-09-28T10:46:56Z
available
2018-09-28T10:46:56Z
issued
2017
citation
GENES(8): 3
issn
2073-4425
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474471.do
Funder
교육부
Funding Program
개인기초연구(교육부)
Project ID
1345270594
Jurisdiction
Rep.of Korea
Project Name
Biosynthesis and releasing mechanism of (neuro)hormones in spawning-induced abalone
rights
openAccess
subject
abalone
Haliotis discus hannai
transcriptome
isoform
PIS system
type
article


Files in This Item

There are no attached files.