Agouti Related Peptide Secreted Via Human Mesenchymal Stem CellsUpregulates Proteasome Activity in an Alzheimer's Disease Model

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474372.do
DOI
10.1038/srep39340
Title
Agouti Related Peptide Secreted Via Human Mesenchymal Stem CellsUpregulates Proteasome Activity in an Alzheimer's Disease Model
Description
This research was supported by the Basic Research Program through theNational Research Foundation of South Korea (NRF) funded by the Ministryof Education (NRF-2014R1A2A1A11050576).
abstract
The activity of the ubiquitin proteasome system (UPS) is downregulated in aggregation diseases such as Alzheimer's disease (AD). In this study, we investigated the therapeutic potential of the Agouti-related peptide (AgRP), which is secreted by human mesenchymal stem cells (MSCs), in terms of its effect on the regulation of proteasome activity in AD. When SH-SY5Y human neuroblastoma cells were co-cultured with MSCs isolated from human Wharton's Jelly (WJ-MSC), their proteasome activity was significantly upregulated. Further analysis of the conditioned media after co-culture allowed us to identify significant concentrations of a neuropeptide, called AgRP. The stereotactic delivery of either WJ-MSCs or AgRP into the hippocampi of C57BL6/J and 5XFAD mice induced a significant increase of proteasome activity and suppressed the accumulation of ubiquitin-conjugated proteins. Collectively, these findings suggest strong therapeutic potential for WJ-MSCs and AgRP to enhance proteasome activity, thereby potentially reducing abnormal protein aggregation and delaying the clinical progression of various neurodegenerative diseases.
provenance
Made available in Cube on 2018-09-28T10:44:19Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Na Kyung
Park, Sang Eon
Kwon, Soo Jin
Shim, Sangmi
Byeon, Yeji
Kim, Jong-Hwa
Na, Duk L.
Chang, Jong Wook
accessioned
2018-09-28T10:44:19Z
available
2018-09-28T10:44:19Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474372.do
Funder
미래창조과학부
Funding Program
개인연구지원
Project ID
1711043293
Jurisdiction
Rep.of Korea
Project Name
Application of mesenchymal stem cells for the screening of therapeutic proteins that will be utilized for the treatment of neurodegenerative diseases
rights
openAccess
type
article


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