Agerarin, identified from Ageratum houstonianum, stimulates circadianCLOCK-mediated aquaporin-3 gene expression in HaCaT keratinocytes

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474362.do
DOI
10.1038/s41598-017-11642-x
Title
Agerarin, identified from Ageratum houstonianum, stimulates circadianCLOCK-mediated aquaporin-3 gene expression in HaCaT keratinocytes
Description
This study was supported by the Basic Science Research Program throughthe National Research Foundation of Korea (NRF) funded by the Ministryof Science, ICT and Future Planning (grant no. 2016R1A2B4008570) and bythe Cooperative Research Program for Agriculture Science & TechnologyDevelopment (project no. PJ011884022016; Rural DevelopmentAdministration), Republic of Korea. This paper was supported by the KUResearch Professor Program of Konkuk University.
abstract
The juice of Ageratum houstonianum is used in folk medicine as an external wound healing aid for skin injuries. However, the active component of A. houstonianum and its mode of action in skin wound healing has not been investigated. This study was conducted to investigate the effect of A. houstonianum ethanolnolic extract (AHE) on the expression of aquaporin-3 (AQP3), an integral membrane protein for water and glycerol transport in keratinocytes, and to identify the structure of the A. houstonianum bioactive compound. Here, we show that AHE increased AQP3 gene expression at the transcriptional level through the p38 MAPK pathway in HaCaT cells. Furthermore, AHE ameliorated suppression of AQP3 expression caused by ultraviolet B (UVB) irradiation. Agerarin (6,7-dimethoxy-2,2-dimethyl-2H-chromene) was identified as the bioactive compound responsible for the up-regulation of AQP3 expression by enhancing the expression of the transcription factor circadian locomotor output cycles kaput (CLOCK). In conclusion, agerarin is a bioactive compound in AHE responsible for CLOCKmediated AQP3 expression in keratinocytes.
provenance
Made available in Cube on 2018-09-28T10:44:03Z (GMT). No. of bitstreams: 0
language
English
author
Shin, Soon Young
Lee, Da Hyun
Gil, Ha-Na
Kim, Beom Soo
Choe, Jeong-Sook
Kim, Jung-Bong
Lee, Young Han
Lim, Yoongho
accessioned
2018-09-28T10:44:03Z
available
2018-09-28T10:44:03Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474362.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274053
Jurisdiction
Rep.of Korea
Project Name
Division of Bioscience and Biotechnology
rights
openAccess
type
article


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