Advanced glycation end-product (AGE)-albumin from activated macrophageis critical in human mesenchymal stem cells survival and post-ischemicreperfusion injury

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474343.do
DOI
10.1038/s41598-017-11773-1
Title
Advanced glycation end-product (AGE)-albumin from activated macrophageis critical in human mesenchymal stem cells survival and post-ischemicreperfusion injury
Description
We thank the FCB-Pharmicell company for providing hBD-MSCs. This studywas supported by a National Research Foundation (no. A121991,2015R1A2A2A01005212; Kang, W. C. and 2017M3A9B402814; Lee, B.) and KoreaHealth Technology R&D Project through the Korea Health IndustryDevelopment Institute, funded by the Ministry of Health & Welfare (no.HI13C2098; Lee, B.) and Ocean Science & Technology Foundation of Korea(no. 20170285; Byun, K.).
abstract
Post-ischemic reperfusion injury (PIRI) triggers an intense inflammatory response which is essential for repair but is also implicated in pathogenesis of post-ischemic remodeling in several organs in human. Stem cell therapy has recently emerged as a promising method for treatment of PIRI in human. However, satisfactory results have not been reported due to severe loss of injected stem cells in PIRI including critical limb ischemia (CLI). For investigating the advanced glycation end-product-albumin (AGE-albumin) from activated macrophages is critical in both muscle cell and stem cell death, we evaluated the recovery of PIRI-CLI by injection of human bone marrow derived mesenchymal stem cells (hBD-MSCs) with or without soluble receptor for AGEs (sRAGE). Our results showed that activated M1 macrophages synthesize and secrete AGE-albumin, which induced the skeletal muscle cell death and injected hBD-MSCs in PIRI-CLI through RAGE increase. Combined injection of sRAGE and hBD-MSCs resulted in enhanced survival of hBD-MSCs and angiogenesis in PIRI-CLI mice. Taken together, AGE-albumin from activated macrophages is critical for both skeletal muscle cell and hBD-MSCs death in PIRI-CLI. Therefore, the inhibition of AGE-albumin from activated macrophages could be a successful therapeutic strategy for treatment of PIRI including CLI with or without stem cell therapy.
provenance
Made available in Cube on 2018-09-28T10:43:32Z (GMT). No. of bitstreams: 0
language
English
author
Son, Myeongjoo
Kang, Woong Chol
Oh, Seyeon
Bayarsaikhan, Delger
Ahn, Hyosang
Lee, Jaesuk
Park, Hyunjin
Lee, Sojung
Choi, Junwon
Lee, Hye Sun
Yang, Phillip C.
Byun, Kyunghee
Lee, Bonghee
accessioned
2018-09-28T10:43:32Z
available
2018-09-28T10:43:32Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474343.do
Funder
보건복지부
Funding Program
첨단의료기술개발
Project ID
1465024158
Jurisdiction
Rep.of Korea
Project Name
Genome-wide Discovery and Functional Studies of the Disease Proteome Encoded in Chromosome 11
rights
openAccess
type
article


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