Adaptor Protein 2 (AP-2) complex is essential for functional axogenesisin hippocampal neurons

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474313.do
DOI
10.1038/srep41620
Title
Adaptor Protein 2 (AP-2) complex is essential for functional axogenesisin hippocampal neurons
Description
We would like to thank Dr. Pablo Ariel for valuable discussions. We alsothank members of the Hoppa lab and The Kim lab for comments. This workis supported by Basic Science Research Program through the NationResearch Foundation of Korea (NRF) funded by the Ministry of science,ICT & Future Planning (NRF-2016M3C7A1905072), and a grant from Kyung HeeUniveristy in 2016 (KHU-20160702) for S.H.K and The Brain ResearchFoundation (BRFSG-2015-05) for I.H.C and M.B.H (Chicago Illinois).
abstract
The complexity and diversity of a neural network requires regulated elongation and branching of axons, as well as the formation of synapses between neurons. In the present study we explore the role of AP-2, a key endocytic adaptor protein complex, in the development of rat hippocampal neurons. We found that the loss of AP-2 during the early stage of development resulted in impaired axon extension and failed maturation of the axon initial segment (AIS). Normally the AIS performs two tasks in concert, stabilizing neural polarity and generating action potentials. In AP-2 silenced axons polarity is established, however there is a failure to establish action potential firing. Consequently, this impairs activity-driven Ca2+ influx and exocytosis at nerve terminals. In contrast, removal of AP-2 from older neurons does not impair axonal growth or signaling and synaptic function. Our data reveal that AP-2 has important roles in functional axogenesis by proper extension of axon as well as the formation of AIS during the early step of neurodevelopment.
provenance
Made available in Cube on 2018-09-28T10:42:44Z (GMT). No. of bitstreams: 0
language
English
author
Kyung, Jae Won
Cho, In Ha
Lee, Sukmook
Song, Woo Keun
Ryan, Timothy A.
Hoppa, Michael B.
Kim, Sung Hyun
orcid
Kim, Sung Hyun/0000-0002-3166-9941
accessioned
2018-09-28T10:42:44Z
available
2018-09-28T10:42:44Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474313.do
Funder
과학기술정보통신부
Funding Program
뇌과학원천기술개발
Project ID
1711048655
Jurisdiction
Rep.of Korea
Project Name
Astrocyte functional changes and regulation of brain function during brain damage and restoration
rights
openAccess
type
article


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