Adaptive evolution of a hyperthermophilic archaeon pinpoints a formatetransporter as a critical factor for the growth enhancement on formate

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474296.do
DOI
10.1038/s41598-017-05424-8
Title
Adaptive evolution of a hyperthermophilic archaeon pinpoints a formatetransporter as a critical factor for the growth enhancement on formate
Description
This work was supported by a grant from the KIOST in-house program(PE99514), the C1 Gas Refinery Program through the National ResearchFoundation of Korea (NRF) funded by the Ministry of Science, ICT andFuture Planning in the Republic of Korea (2015M3D3A1A01064884), and theDevelopment of Technology for Biohydrogen Production UsingHyperthermophilic Archaea program of the Ministry of Oceans andFisheries in the Republic of Korea.
abstract
Previously, we reported that the hyperthermophilic archaeon Thermococcus onnurineus NA1 could grow on formate and produce H-2. Formate conversion to hydrogen was mediated by a formate-hydrogen lyase complex and was indeed a part of chemiosmotic coupling to ATP generation. In this study, we employed an adaptation approach to enhance the cell growth on formate and investigated molecular changes. As serial transfer continued on formate-containing medium at the serum vial, cell growth, H2 production and formate consumption increased remarkably. The 156 times transferred-strain, WTF156T, was demonstrated to enhance H2 production using formate in a bioreactor. The whole-genome sequencing of the WTF-156T strain revealed eleven mutations. While no mutation was found among the genes encoding formate hydrogen lyase, a point mutation (G154A) was identified in a formate transporter (TON_1573). The TON_1573 (A52T) mutation, when introduced into the parent strain, conferred increase in formate consumption and H2 production. Another adaptive passage, carried out by culturing repeatedly in a bioreactor, resulted in a strain, which has a mutation in TON_1573 (C155A) causing amino acid change, A52E. These results implicate that substitution of A52 residue of a formate transporter might be a critical factor to ensure the increase in formate uptake and cell growth.
provenance
Made available in Cube on 2018-09-28T10:42:17Z (GMT). No. of bitstreams: 0
language
English
author
Jung, Hae-Chang
Lee, Seong Hyuk
Lee, Sung-Mok
An, Young Jun
Lee, Jung-Hyun
Lee, Hyun Sook
Kang, Sung Gyun
accessioned
2018-09-28T10:42:17Z
available
2018-09-28T10:42:17Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474296.do
Funder
해양수산부
Funding Program
한국해양과학기술원연구운영비지원
Project ID
1525007361
Jurisdiction
Rep.of Korea
Project Name
비광합성 해양·극한 미생물의 탄소 대사과정 규명 및 활용
rights
openAccess
type
article


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