Activation of intestinal olfactory receptor stimulates glucagon-likepeptide-1 secretion in enteroendocrine cells and attenuateshyperglycemia in type 2 diabetic mice

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474236.do
DOI
10.1038/s41598-017-14086-5
Title
Activation of intestinal olfactory receptor stimulates glucagon-likepeptide-1 secretion in enteroendocrine cells and attenuateshyperglycemia in type 2 diabetic mice
Description
We are grateful to Dr. Robert F. Margolskee (Monell Chemical SensesCenter, Philadelphia, PA, USA) for providing us with critical readingand opinion on our manuscript. This research was supported by the BasicScience Research Program through the National Research Foundation ofKorea (NRF), which is funded by the Ministry of Education, Science andTechnology (20131A1A2004960, 2017R1A2B4003422).
abstract
Odorants are non-nutrients. However, they exist abundantly in foods, wines, and teas, and thus can be ingested along with the other nutrients during a meal. Here, we have focused on the chemical-recognition ability of these ORs and hypothesized that the odorants ingested during a meal may play a physiological role by activating the gut-expressed ORs. Using a human-derived enteroendocrine L cell line, we discovered the geraniol-and citronellal-mediated stimulation of glucagon-like peptide-1 (GLP1) secretion and elucidated the corresponding cellular downstream signaling pathways. The geraniol-stimulated GLP-1 secretion event in the enteroendocrine cell line was mediated by the olfactory-type G protein, the activation of adenylyl cyclase, increased intracellular cAMP levels, and extracellular calcium influx. TaqMan qPCR demonstrated that two ORs corresponding to geraniol and citronellal were expressed in the human enteroendocrine cell line and in mouse intestinal specimen. In a type 2 diabetes mellitus mouse model (db/db), oral administration of geraniol improved glucose homeostasis by increasing plasma GLP-1 and insulin levels. This insulinotropic action of geraniol was GLP-1 receptor-mediated, and also was glucose-dependent. This study demonstrates that odor compounds can be recognized by gut-expressed ORs during meal ingestion and therefore, participate in the glucose homeostasis by inducing the secretion of gut-peptides.
provenance
Made available in Cube on 2018-09-28T10:40:46Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Ki-Suk
Lee, In-Seung
Kim, Kang-Hoon
Park, Jiyoung
Kim, Yumi
Choi, Jeong-Hee
Choi, Jin-Sung
Jang, Hyeung-Jin
orcid
Choi, Jin-Sung/0000-0002-8439-6035
accessioned
2018-09-28T10:40:46Z
available
2018-09-28T10:40:46Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474236.do
Funder
교육부
Funding Program
이공학개인기초연구지원
Project ID
1345236380
Jurisdiction
Rep.of Korea
Project Name
Study on the incretin secretion mechanism of herbal essential oil through gut-expressed olfactory receptor
rights
openAccess
type
article


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