Accelerated oral wound healing using a pre-vascularized mucosal cellsheet

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474179.do
DOI
10.1038/s41598-017-10991-x
Title
Accelerated oral wound healing using a pre-vascularized mucosal cellsheet
Description
This study was supported by two grants (no. HI15C2920 and HI14C23050000)from the Korean Health Technology R&D Project through the Korea HealthIndustry Development Institute (KHIDI), Ministry of Health & Welfare,Seoul, Republic of Korea (J.-L. Roh).
abstract
Cell sheets with pre-vascularization have recently been developed but remain relatively untested in oral wound healing. Therefore, we examined the potential utility of our newly developed pre-vascularized mucosal cell sheets in oral wound healing. Mucosal keratinocytes, fibroblasts, and endothelial progenitor cells were primarily cultured for in vitro cell expansion from mucosa and blood of Sprague-Dawley rats. Mucosal cell sheets were generated using cultured keratinocytes and plasma fibrin (K sheet) or keratinocytes and a mixture of fibrin, fibroblasts, and endothelial cells (PV sheet). Autologous sheets were transplanted on deep wounds in the buccal region of rats. The gross and histological characteristics of wound healing were compared among control wound, K sheet, and PV sheet groups. We successfully cultured and expanded keratinocytes, fibroblasts, and endothelial progenitor cells in vitro for generating mucosal cell sheets with or without pre-vascularization. In the in vivo oral wound model, compared with the control wound, the PV sheet group exhibited rapid wound closure more prominently than the K sheet group. The histological healing in the PV sheet group was similar to that in rat normal buccal mucosa without fibrosis. The pre-vascularized mucosal cell sheet exhibited in vivo efficacy in oral wound healing by promoting accelerated healing.
provenance
Made available in Cube on 2018-09-28T10:39:16Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Jaewang
Kim, Eun Hye
Shin, Daiha
Roh, Jong-Lyel
orcid
Roh, Jong-Lyel/0000-0002-1537-1977
accessioned
2018-09-28T10:39:16Z
available
2018-09-28T10:39:16Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474179.do
Funder
보건복지부
Funding Program
첨단의료기술개발
Project ID
1465021367
Jurisdiction
Rep.of Korea
Project Name
Development of 3-D mucosal cell sheet production and autotransplantation technology for treatment of mucosal defects in the upper aerodigestive tract
rights
openAccess
type
article


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