A tyrosinase, mTyr-CNK, that is functionally available as a monophenolmonooxygenase

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474120.do
DOI
10.1038/s41598-017-17635-0
Title
A tyrosinase, mTyr-CNK, that is functionally available as a monophenolmonooxygenase
Description
This work was supported by the Marine Biomaterials Research Center grantfrom the Marine Biotechnology Program of the Korea Institute of MarineScience & Technology Promotion funded by the Ministry of Oceans andFisheries, Korea (to H.J. Cha & Y.S. Choi) and was also supported by theC1 Gas Refinery Program of the National Research Foundation of Korea(NRF), which was funded by the Ministry of Science, ICT & FuturePlanning (NRF-2016M3D3A1A01913258, to Y.S. Choi).
abstract
Tyrosinase efficiently catalyzes the ortho-hydroxylation of monophenols and the oxidation of diphenols without any additional cofactors. Although it is of significant interest for the biosynthesis of catechol derivatives, the rapid catechol oxidase activity and inactivation of tyrosinase have hampered its practical utilization as a monophenol monooxygenase. Here, we prepared a functional tyrosinase that exhibited a distinguished monophenolase/diphenolase activity ratio (V-max mono/V-max di = 3.83) and enhanced catalytic efficiency against L-tyrosine (k(cat) = 3.33 +/- 0.18 s(-1), K-m = 2.12 +/- 0.14 mM at 20 degrees C and pH 6.0). This enzyme was still highly active in ice water (>80%), and its activity was well conserved below 30 degrees C. In vitro DOPA modification, with a remarkably high yield as a monophenol monooxygenase, was achieved by the enzyme taking advantage of these biocatalytic properties. These results demonstrate the strong potential for this enzyme's use as a monophenol monooxygenase in biomedical and industrial applications.
provenance
Made available in Cube on 2018-09-28T10:37:44Z (GMT). No. of bitstreams: 0
language
English
author
Do, Hyunsu
Kang, Eungsu
Yang, Byeongseon
Cha, Hyung Joon
Choi, Yoo Seong
accessioned
2018-09-28T10:37:44Z
available
2018-09-28T10:37:44Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474120.do
Funder
해양수산부
Funding Program
해양수산생명공학기술개발
Project ID
1525006929
Jurisdiction
Rep.of Korea
Project Name
해양 바이오미네랄 기반 기능성 복합 골이식재 개발
rights
openAccess
type
article


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