A simple method to control glycolytic flux for the design of an optimalcell factory

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/474007.do
DOI
10.1186/s13068-017-0847-4
Title
A simple method to control glycolytic flux for the design of an optimalcell factory
Description
This research was supported by grants from the Advanced Biomass R&DCenter (ABC) of Global Frontier Project (ABC-2015M3A6A2066119) and C1Gas Refinery Program (NRF-2016M3D3A1A01913237) through the NationalResearch Foundation of Korea (NRF) funded by the Ministry of Science,ICT and Future Planning, Korea.
abstract
Background: A microbial cell factory with high yield and productivity are prerequisites for an economically feasible bio-based chemical industry. However, cell factories that show a kinetic imbalance between glycolysis and product formation pathways are not optimal. Glycolysis activity is highly robust for survival in nature, but is not optimized for chemical production.
Results: Here, we propose a novel approach to balance glycolytic activity with the product formation capacity by precisely controlling expression level of ptsG (encoded glucose transporter) through UTR engineering. For various heterologous pathways with different maximum production rates, e.g., n-butanol, butyrate, and 2,3-butanediol, glycolytic fluxes could be successfully modulated to maximize yield and productivity, while minimizing by-product formation in Escherichia coli.
Conclusions: These results support the application of this simple method to explore the maximum yield and productivity when designing optimal cell factories for value-added products in the fields of metabolic engineering and synthetic biology.
provenance
Made available in Cube on 2018-09-28T10:34:44Z (GMT). No. of bitstreams: 0
language
English
author
Lim, Jae Hyung
Jung, Gyoo Yeol
orcid
Jung, Gyoo Yeol/0000-0002-9742-3207
accessioned
2018-09-28T10:34:44Z
available
2018-09-28T10:34:44Z
issued
2017
citation
BIOTECHNOLOGY FOR BIOFUELS(10)
issn
1754-6834
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/474007.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274025
Jurisdiction
Rep.of Korea
Project Name
program for advanced education of creative Chemical Engineers
rights
openAccess
subject
Glycolytic flux
n-Butanol
2,3-Butanediol
Butyrate
UTR engineering
PTS
type
article


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