A ring-polymer model shows how macromolecular crowding controlschromosome-arm organization in Escherichia coli

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473965.do
DOI
10.1038/s41598-017-10421-y
Title
A ring-polymer model shows how macromolecular crowding controlschromosome-arm organization in Escherichia coli
Description
BYH acknowledges useful discussions with S. Jun. This work was supportedby the collaborative research contract funded by Korea Institute ofScience and Technology Information (KISTI) and NSERC (Canada) (B-YH) aswell as by the Basic Science Research Program through the grant no.2015R1D1A1A09057469 (YJ). We acknowledge the computational resources ofthe Shared Hierarchical Academic Research Computing Network (SHARCNET:www.sharcnet.ca) and Compute/Calcul Canada.
abstract
Macromolecular crowding influences various cellular processes such as macromolecular association and transcription, and is a key determinant of chromosome organization in bacteria. The entropy of crowders favors compaction of long chain molecules such as chromosomes. To what extent is the circular bacterial chromosome, often viewed as consisting of "two arms", organized entropically by crowding? Using computer simulations, we examine how a ring polymer is organized in a crowded and cylindrically-confined space, as a coarse-grained bacterial chromosome. Our results suggest that in a wide parameter range of biological relevance crowding is essential for separating the two arms in the way observed with Escherichia coli chromosomes at fast-growth rates, in addition to maintaining the chromosome in an organized collapsed state. Under different conditions, however, the ring polymer is centrally condensed or adsorbed onto the cylindrical wall with the two arms laterally collapsed onto each other. We discuss the relevance of our results to chromosome-membrane interactions.
provenance
Made available in Cube on 2018-09-28T10:33:38Z (GMT). No. of bitstreams: 0
language
English
author
Jeon, Chanil
Jung, Youngkyun
Ha, Bae-Yeun
accessioned
2018-09-28T10:33:38Z
available
2018-09-28T10:33:38Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473965.do
Funder
과학기술정보통신부
Funding Program
한국고등과학원연구운영비지원
Project ID
1711061716
Jurisdiction
Rep.of Korea
Project Name
Advanced & Basic Science Research
rights
openAccess
type
article


Files in This Item

There are no attached files.