A Phytochemical Approach to Promotion of Self-renewal in MurineSpermatogonial Stem Cell by Using Sedum Sarmentosum Extract

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473896.do
DOI
10.1038/s41598-017-11790-0
Title
A Phytochemical Approach to Promotion of Self-renewal in MurineSpermatogonial Stem Cell by Using Sedum Sarmentosum Extract
Description
This research was supported by grants from the KRIBB (Korea ResearchInstitute of Bioscience and Biotechnology) Research Initiative Program(KGM424743) and Next-Generation BioGreen 21 Program (PJ011347), RuralDevelopment Administration, Republic of Korea.
abstract
Spermatogonial stem cells (SSCs) are the basis of spermatogenesis, which is dependent on the ability to self-renew and differentiation. Controlling self-renewal and differentiation of SSCs could apply to treatment of disease such as male infertility. Recently, in the field of stem cell research, it was demonstrated that effective increase in stem cell activity can be achieved by using growth factors derived from plant extracts. In this study, our aim is to investigate components from natural plant to improve the self-renewal of SSCs. To find the components, germ cells were cultured with comprehensive natural plant extracts, and then the more pure fraction, and finally single compound at different concentrations. As a result, we found 5H-purin-6-amine at 1 mu g/mL, originated from Sedum sarmentosum, was a very effective compound induced SSCs proliferation. Our data showed that germ cells cultured with 5H-purin-6-amine could maintain their stable characteristics. Furthermore, transplantation results demonstrated that 5H-purin-6-amine at 1 mu g/mL increased the activity of SSCs, indicating the compound could increase true SSC concentration within germ cells to 1.96-fold. These findings would be contributed to improve further reproductive research and treat male infertility by using natural plant extracts.
provenance
Made available in Cube on 2018-09-28T10:31:46Z (GMT). No. of bitstreams: 0
language
English
author
Jung, Sang-Eun
Kim, Yong-Hee
Cho, Sunghun
Kim, Bang-Jin
Lee, Hee-Seok
Hwang, Seongsoo
Kim, Geun-Bae
Kim, Young-Hyun
Pang, Myung-Geol
Lee, Sanghyun
Ryu, Buom-Yong
orcid
HWANG, Seongsoo/0000-0002-0512-7049
accessioned
2018-09-28T10:31:46Z
available
2018-09-28T10:31:46Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473896.do
Funder
과학기술정보통신부
Funding Program
한국생명공학연구원연구운영비지원
Project ID
1711063660
Jurisdiction
Rep.of Korea
Project Name
National Primate Research Center Project
rights
openAccess
type
article


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