A patinib-loaded nanoparticles suppress vascular endothelial growthfactor-induced angiogenesis and experimental corneal neovascularization

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473885.do
DOI
10.2147/IJN.S135133
Title
A patinib-loaded nanoparticles suppress vascular endothelial growthfactor-induced angiogenesis and experimental corneal neovascularization
Description
This research was supported by the Bio & Medical Technology DevelopmentProgram of National Research Foundation of Korea grants funded by theKorea government (2012M3A9C6050368, 2015R1D1A1A02061724, and2016R1A2B4015056).
abstract
Pathological angiogenesis is one of the major symptoms of severe ocular diseases, including corneal neovascularization. The blockade of vascular endothelial growth factor (VEGF) action has been recognized as an efficient strategy for treating corneal neovascularization. In this study, we aimed to investigate whether nanoparticle-based delivery of apatinib, a novel and selective inhibitor of VEGF receptor 2, inhibits VEGF-mediated angiogenesis and suppresses experimental corneal neovascularization. Water-insoluble apatinib was encapsulated in nanoparticles composed of human serum albumin (HSA)-conjugated polyethylene glycol (PEG). In vitro angiogenesis assays showed that apatinib-loaded HSA-PEG (Apa-HSA-PEG) nanoparticles potently inhibited VEGF-induced tube formation, scratch wounding migration, and proliferation of human endothelial cells. In a rat model of alkali burn injury-induced corneal neovascularization, a subconjunctival injection of Apa-HSA-PEG nanoparticles induced a significant decrease in neovascularization compared to that observed with an injection of free apatinib solution or phosphate-buffered saline. An in vivo distribution study using HSA-PEG nanoparticles loaded with fluorescent hydrophobic model drugs revealed the presence of a substantial number of nanoparticles in the corneal stroma within 24 h after injection. These in vitro and in vivo results demonstrate that apatinib-loaded nanoparticles may be promising for the prevention and treatment of corneal neovascularization-related ocular disorders.
provenance
Made available in Cube on 2018-09-28T10:31:28Z (GMT). No. of bitstreams: 0
language
English
author
Lee, Jung Eun
Kim, Koung Li
Kim, Danbi
Yeo, Yeongju
Han, Hyounkoo
Kim, Myung Goo
Kim, Sun Hwa
Kim, Hyuncheol
Jeong, Ji Hoon
Suh, Wonhee
accessioned
2018-09-28T10:31:28Z
available
2018-09-28T10:31:28Z
issued
2017
citation
INTERNATIONAL JOURNAL OF NANOMEDICINE(12)
issn
1178-2013
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473885.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274276
Jurisdiction
Rep.of Korea
Project Name
Micro-scale controlled biochemical engineering process
rights
openAccess
subject
apatinib
corneal neovascularization
nanoparticle
vascular endothelialgrowth factor
type
article


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