A Paper-Based Device for Performing Loop-Mediated IsothermalAmplification with Real-Time Simultaneous Detection of Multiple DNATargets

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473880.do
DOI
10.7150/thno.18675
Title
A Paper-Based Device for Performing Loop-Mediated IsothermalAmplification with Real-Time Simultaneous Detection of Multiple DNATargets
Description
This work was financially supported by the new industry creation projectof the National Research Foundation of Korea (NRF) (NRF-2013M3C1A8A01072759), the R&D Joint Venture Program (2015K000184) of the NRF, theOriginal Technology Research Program for Brain Science through theNational Research Foundation of Korea (NRF) (NRF-2015M3C7A1029196).
abstract
Paper-based diagnostic devices have many advantages as a one of the multiple diagnostic test platforms for point-of-care (POC) testing because they have simplicity, portability, and cost-effectiveness. However, despite high sensitivity and specificity of nucleic acid testing (NAT), the development of NAT based on a paper platform has not progressed as much as the others because various specific conditions for nucleic acid amplification reactions such as pH, buffer components, and temperature, inhibitions from technical differences of paper-based device. Here, we propose a paper-based device for performing loop-mediated isothermal amplification (LAMP) with real-time simultaneous detection of multiple DNA targets. We determined the optimal chemical components to enable dry conditions for the LAMP reaction without lyophilization or other techniques. We also devised the simple paper device structure by sequentially stacking functional layers, and employed a newly discovered property of hydroxynaphthol blue fluorescence to analyze real-time LAMP signals in the paper device. This proposed platform allowed analysis of three different meningitis DNA samples in a single device with single-step operation. This LAMP-based multiple diagnostic device has potential for real-time analysis with quantitative detection of 10(2)-10(5) copies of genomic DNA. Furthermore, we propose the transformation of DNA amplification devices to a simple and affordable paper system approach with great potential for realizing a paper-based NAT system for POC testing.
provenance
Made available in Cube on 2018-09-28T10:31:20Z (GMT). No. of bitstreams: 0
language
English
author
Seok, Youngung
Joung, Hyou-Arm
Byun, Ju-Young
Jeon, Hyo-Sung
Shin, Su Jeong
Kim, Sanghyo
Shin, Young-Beom
Han, Hyung Soo
Kim, Min-Gon
accessioned
2018-09-28T10:31:20Z
available
2018-09-28T10:31:20Z
issued
2017
citation
THERANOSTICS(7): 8
issn
1838-7640
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473880.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345273940
Jurisdiction
Rep.of Korea
Project Name
Center for Advanced Photon Science
rights
openAccess
subject
loop-mediated isothermal amplification
paper
biosensor
moleculardiagnosis
nucleic acid testing
point-of-care
type
article


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