A novel Salmonella strain inactivated by a regulated autolysis systemand expressing the B subunit of Shiga toxin 2e efficiently elicitsimmune responses and confers protection against virulent Stx2e-producingEscherichia coli

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473862.do
DOI
10.1186/s12917-017-0962-2
Title
A novel Salmonella strain inactivated by a regulated autolysis systemand expressing the B subunit of Shiga toxin 2e efficiently elicitsimmune responses and confers protection against virulent Stx2e-producingEscherichia coli
Description
This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government (MISP) (No.2015R1A2A1A14001011).
abstract
Background: Salmonella Typhimurium (S. Typhimurium) inactivated by a regulated autolysis system was genetically engineered to express the homo-pentameric B subunit of Shiga toxin 2e (Stx2eB) on its surface. To prepare a strain able to yield autolyzed Salmonella bearing Stx2eB, the plasmid pJHL184 harboring stx(2eB) gene was transformed into the attenuated S. Typhimurium strain, JOL1454. Stx2eB subcloned into the antigen delivery cassette of the plasmid was expressed as fusion protein with the outer membrane protein
Results: The expression of Stx2eB fused to the signal peptide in JOL1454 was validated by immunoblot analysis. To determine the immunogenicity of JOL1454, female BALB/c mice were intramuscularly injected with 1 x 10(8) CFU of the inactivated cells at weeks 0 and 2. Significantly elevated levels of IgG and IgA specific to Stx2eB was observed at weeks 4 and 6 post-immunization (PI) (P < 0.05). Proportion of CD (broken vertical bar) CD (broken vertical bar) T lymphocyte subpopulation was also significantly augmented in in vivo stimulated splenocytes relative to that in the control group. The increased titers of IgG1 and IgG2a, and of immunomodulatory cytokines indicated that the immunization elicited Th1 and Th2 immune responses. Further, immunomodulatory cytokine genes (IL-6, IL-17A, IL21 and JOL1454) efficiently upregulated in naive porcine peripheral blood mononuclear cells (PBMCs) pulsed with JOL1454. At week 6 PI, following the challenge with a virulent Stx2e-producing Escherichia coli in the mice, all immunized mice survived whereas approximately 30% of the mice in the control group died.
Conclusions: JOL1454 provided superior immunogenicity and effective protection against challenge with a sublethal dose, which demonstrates its potential as a candidate vaccine against edema disease.
provenance
Made available in Cube on 2018-09-28T10:30:51Z (GMT). No. of bitstreams: 0
language
English
author
Won, Gayeon
Kim, Tae Hoon
Lee, John Hwa
accessioned
2018-09-28T10:30:51Z
available
2018-09-28T10:30:51Z
issued
2017
citation
BMC VETERINARY RESEARCH(13)
issn
1746-6148
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473862.do
Funder
과학기술정보통신부
Funding Program
개인기초연구(미래부)
Project ID
1711052289
Jurisdiction
Rep.of Korea
Project Name
Development of vaccines for infectious diseases using novel concept delivery system and vector plasmid for effective antigen secretion
rights
openAccess
subject
Shiga toxin
Edema disease
Bacterial ghost
Salmonella typhimurium
type
article


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