A novel model of THO/TREX loading onto target RNAs in metazoan geneexpression

Collection with item attached
2016
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473847.do
DOI
10.5483/BMBRep.2016.49.7.099
Title
A novel model of THO/TREX loading onto target RNAs in metazoan geneexpression
Description
This work was supported by grants from the Basic Science ResearchProgram through the National Research Foundation of Korea (NRF) (no.2015R1A2A2A01003598) to YDC.
abstract
The THO/TREX complex consists of several conserved subunits and is required for mRNA export. In metazoans, THO/TREX binds a subset of mRNAs during RNA splicing, and facilitates their nuclear export. How THO/TREX selects RNA targets is, however, incompletely understood. In our recent study, we reported that THO is loaded onto Piwi-interacting RNA (piRNA) precursor transcripts independent of splicing, and facilitates convergent transcription in Drosophila ovary. The precursors are later processed into mature piRNAs, small noncoding RNAs that silence transposable elements (TEs). We observed that piRNAs originating from dual-strand clusters, where precursors are transcribed from both strands, were specifically affected by THO mutation. Analysis of THO-bound RNAs showed enrichment of dual-strand cluster transcripts. Interestingly, THO loading onto piRNA precursors was dependent on Cutoff (Cuff), which comprises the Rhino-Deadlock-Cutoff (RDC) complex that is recruited to dual-strand clusters by recognizing H3K9me3 and licenses convergent transcription from he cluster. We also found that THO mutation affected transcription from dual-strand clusters. Therefore, we concluded that THO/TREX is recruited to dual-strand piRNA clusters, independent of splicing events, via multi-protein interactions with chromatin structure. Then, it facilitates transcription likely by suppressing premature termination to ensure adequate expression of piRNA precursors.
provenance
Made available in Cube on 2018-09-28T10:30:26Z (GMT). No. of bitstreams: 0
language
English
author
Hur, Junho K.
Chung, Yun Doo
accessioned
2018-09-28T10:30:26Z
available
2018-09-28T10:30:26Z
issued
2016
citation
BMB REPORTS(49): 7
issn
1976-6696
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473847.do
Funder
미래창조과학부
Funding Program
개인연구지원
Project ID
1711036001
Jurisdiction
Rep.of Korea
Project Name
Study on the role of Drosophila THO in the biogensis of piRNA and regulation of germline stem cells
rights
openAccess
subject
Drosophila
Dual-strand piRNA cluster
piRNA
RDC(Rhino-Deadlock-Cutoff) complex
THO/TREX
type
article


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