A novel bZIP protein, Gsb1, is required for oxidative stress response,mating, and virulence in the human pathogen Cryptococcus neoformans

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473818.do
DOI
10.1038/s41598-017-04290-8
Title
A novel bZIP protein, Gsb1, is required for oxidative stress response,mating, and virulence in the human pathogen Cryptococcus neoformans
Description
This work was supported by the National Research Foundation of Korea(Grant No. NRF2016R1D1A1B03934249) from the Korean Ministry of Ministryof Science, ICT and Future Planning and by the Strategic Initiative forMicrobiomes in Agriculture and Food from the Korean Ministry ofAgriculture, Food, and Rural Affairs (Grant No. 914007-4) to H. A. K.This work was partly supported by the Strategic Initiative forMicrobiomes in Agriculture and Food funded by Ministry of Agriculture,Food, and Rural Affairs (Grant No. 916006-2) to Y.-S. B. We are gratefulto Dr. Yin-Won Lee for providing inspiring comments and encouragement.
abstract
The human pathogen Cryptococcus neoformans, which causes life-threatening meningoencephalitis in immunocompromised individuals, normally faces diverse stresses in the human host. Here, we report that a novel, basic, leucine-zipper (bZIP) protein, designated Gsb1 (general stress-related bZIP protein 1), is required for its normal growth and diverse stress responses. C. neoformans gsb1 Delta mutants grew slowly even under non-stressed conditions and showed increased sensitivity to high or low temperatures. The hypersensitivity of gsb1 Delta to oxidative and nitrosative stresses was reversed by addition of a ROS scavenger. RNA-Seq analysis during normal growth revealed increased expression of a number of genes involved in mitochondrial respiration and cell cycle, but decreased expression of several genes involved in the mating-pheromone-responsive MAPK signaling pathway. Accordingly, gsb1 Delta showed defective mating and abnormal cell-cycle progression. Reflecting these pleiotropic phenotypes, gsb1 Delta exhibited attenuated virulence in a murine model of cryptococcosis. Moreover, RNA-Seq analysis under oxidative stress revealed that several genes involved in ROS defense, cell-wall remodeling, and protein glycosylation were highly induced in the wild-type strain but not in gsb1 Delta. Gsb1 localized exclusively in the nucleus in response to oxidative stress. In conclusion, Gsb1 is a key transcription factor modulating growth, stress responses, differentiation, and virulence in C. neoformans.
provenance
Made available in Cube on 2018-09-28T10:29:39Z (GMT). No. of bitstreams: 0
language
English
author
Cheon, Seon Ah
Thak, Eun Jung
Bahn, Yong-Sun
Kang, Hyun Ah
orcid
Bahn, Yong-Sun/0000-0001-9573-5752
accessioned
2018-09-28T10:29:39Z
available
2018-09-28T10:29:39Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473818.do
Funder
농림축산식품부
Funding Program
포스트게놈신산업육성을위한다부처유전체사업(농림부)
Project ID
1545015509
Jurisdiction
Rep.of Korea
Project Name
한국 전통주류 발효효모 균주의 참조유전체 및 오믹스 분석 연구
rights
openAccess
type
article


Files in This Item

There are no attached files.