A monoclonal antibody recognizes undifferentiation-specific carbohydratemoieties expressed on cell surface of the human dental pulp cells

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473744.do
DOI
10.1016/j.scr.2017.04.002
Title
A monoclonal antibody recognizes undifferentiation-specific carbohydratemoieties expressed on cell surface of the human dental pulp cells
Description
This research was supported by the Bio & Medical Technology DevelopmentProgram of the NRF funded by the Korean government, MSIP(NRF-2015M3A9C6029130).
abstract
Human dental pulp cells are obtained fromdental pulp tissue, and have the ability to formdentin and a pulp-like complex. Although adult stem cells have been identified from the primary culture by using specific cell surface markers, the identity of surface markers for the purification of stem cells within the dental pulp population are still unclear. Previously, we had constructed monoclonal antibodies against the undifferentiated cell-specific surface markers of human dental pulp cells (hDPCs) by performing decoy immunization. Among them, a monoclonal antibody against the cell surface antigen of the undifferentiated hDPCs (named UPSA-1) was purified and its heavy and light chain consensus regionswere analyzed. The cell surface binding affinity of UPSA-1mAb on the undifferentiated hDPCs was stronger than that on the differentiated cells. When tunicamycin was applied to hDPSCs during culture, the cell surface binding affinity of the antibody was dramatically decreased, and dentinogenic differentiation was reduced. The purified UPSA-1 antigen band resulting from immunoprecipitation disappeared or shifted down on the SDS-PAGE by deglycosylation. These data suggested that glycosylation on the cell surface might be a marker of an undifferentiated state, and that UPSA-1 mAb might be useful for identifying the carbohydrate moiety on the cell surface of undifferentiated pulp cells. (C) 2017 The Authors. Published by Elsevier B.V.
provenance
Made available in Cube on 2018-09-28T10:27:43Z (GMT). No. of bitstreams: 0
language
English
author
Kang, Kyung-Jung
Ko, Seon-Yle
Ryu, Chun-Jeih
Jang, Young-Joo
accessioned
2018-09-28T10:27:43Z
available
2018-09-28T10:27:43Z
issued
2017
citation
STEM CELL RESEARCH(21)
issn
1873-5061
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473744.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274174
Jurisdiction
Rep.of Korea
Project Name
Epigenetic regulation of transcriptional signal pathway
rights
openAccess
subject
Human dental pulp cells (hDPCs)
Undifferentiation-specific surfacemarker
Glycosylation
type
article


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