A Microfluidic Immunostaining System Enables Quality Assured andStandardized Immunohistochemical Biomarker Analysis

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473725.do
DOI
10.1038/srep45968
Title
A Microfluidic Immunostaining System Enables Quality Assured andStandardized Immunohistochemical Biomarker Analysis
Description
This research was supported by a grant from the National Cancer Center,Korea (1410691) and by a Mid-Career Researcher Program(NRF-2016R1A2B3015986) and a Bio & Medical Technology DevelopmentProgram (NRF-2015M3A9B3028685) through the National Research Foundationof Korea funded by the Ministry of Science, ICT and Future Planning. Theauthors also acknowledge a KAIST Systems Healthcare Program.
abstract
Immunohistochemistry (IHC) plays an important role in biomarker-driven cancer therapy. Although there has been a high demand for standardized and quality assured IHC, it has rarely been achieved due to the complexity of IHC testing and the subjective validation-based process flow of IHC quality control. We present here a microfluidic immunostaining system for the standardization of IHC by creating a microfluidic linearly graded antibody (Ab)-staining device and a reference cell microarray. Unlike conventional efforts, our system deals primarily with the screening of biomarker staining conditions for quantitative quality assurance testing in IHC. We characterized the microfluidic matching of Ab staining intensity using three HER2 Abs produced by different manufacturers. The quality of HER2 Ab was also validated using tissues of breast cancer patients, demonstrating that our system is an efficient and powerful tool for the standardization and quality assurance of IHC.
provenance
Made available in Cube on 2018-09-28T10:27:13Z (GMT). No. of bitstreams: 0
language
English
author
Kwon, Seyong
Cho, Chang Hyun
Kwon, Youngmee
Lee, Eun Sook
Park, Je-Kyun
orcid
Park, Je-Kyun/0000-0003-4522-2574
accessioned
2018-09-28T10:27:13Z
available
2018-09-28T10:27:13Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473725.do
Funder
과학기술정보통신부
Funding Program
한국과학기술원연구운영비지원(0.5)
Project ID
1711063537
Jurisdiction
Rep.of Korea
Project Name
Systems Healthcare
rights
openAccess
type
article


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