A Low Permeability Microfluidic Blood-Brain Barrier Platform with DirectContact between Perfusable Vascular Network and Astrocytes

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473698.do
DOI
10.1038/s41598-017-07416-0
Title
A Low Permeability Microfluidic Blood-Brain Barrier Platform with DirectContact between Perfusable Vascular Network and Astrocytes
Description
I would like to acknowledge James Yu for his proofreading and editing ofthe final draft of this publication. This work was supported by theNational Research Foundation of Korea (NRF; Grant No.2015R1A2A1A09005662, 2014M3C7A1074040, 2016R1A4A1010796 and2016M3A9B4917321).
abstract
A novel three dimensional blood brain barrier (BBB) platform was developed by independently supplying different types of media to separate cell types within a single device. One channel (vascular channel, VC) is connected to the inner lumen of the vascular network while the other supplies media to the neural cells (neural channel, NC). Compared to co-cultures supplied with only one type of medium (or 1: 1 mixture), best barrier properties and viability were obtained with culturing HUVECs with endothelial growth medium (EGM) and neural cells with neurobasal medium supplemented with fetal bovine serum (NBMFBS) independently. The measured vascular network permeability were comparable to reported in vivo values (20 kDa FITC-dextran, 0.45 +/- 0.11 x 10(-6) cm/s; 70 kDa FITC-dextran, 0.36 +/- 0.05 x 10(-6) cm/s) and a higher degree of neurovascular interfacing (astrocytic contact with the vascular network, GFAP-CD31 stain overlap) and presence of synapses (stained with synaptophysin). The BBB platform can dependably imitate the perivascular network morphology and synaptic structures characteristic of the NVU. This microfluidic BBB model can find applications in screening pharmaceuticals that target the brain for in neurodegenerative diseases.
provenance
Made available in Cube on 2018-09-28T10:26:30Z (GMT). No. of bitstreams: 0
language
English
author
Bang, Seokyoung
Lee, Seung-Ryeol
Ko, Jihoon
Son, Kyungmin
Tahk, Dongha
Ahn, Jungho
Im, Changkyun
Jeon, Noo Li
accessioned
2018-09-28T10:26:30Z
available
2018-09-28T10:26:30Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473698.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274022
Jurisdiction
Rep.of Korea
Project Name
BK21+ Transformative Training Program for Creative Mechanical and Aerospace Engineers
rights
openAccess
type
article


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