A Lipid Emulsion Reverses Toxic-Dose Bupivacaine-Induced Vasodilationduring Tyrosine Phosphorylation-Evoked Contraction in Isolated RatAortae

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473686.do
DOI
10.3390/ijms18020394
Title
A Lipid Emulsion Reverses Toxic-Dose Bupivacaine-Induced Vasodilationduring Tyrosine Phosphorylation-Evoked Contraction in Isolated RatAortae
Description
This research was supported by Basic Science Research Program throughthe National Research Foundation of Korea (NRF) funded by the Ministryof Education (NRF-2016R1D1A1B03930451).
abstract
The goal of this in vitro study was to examine the effect of a lipid emulsion on toxic-dose bupivacaine-induced vasodilation in a model of tyrosine phosphatase inhibitor sodium orthovanadate-induced contraction in endothelium-denuded rat aortae and to elucidate the associated cellular mechanism. The effect of a lipid emulsion on vasodilation induced by a toxic dose of a local anesthetic during sodium orthovanadate-induced contraction was examined. In addition, the effects of various inhibitors, either bupivacaine alone or a lipid emulsion plus bupivacaine, on protein kinase phosphorylation induced by sodium orthovanadate in rat aortic vascular smooth muscle cells was examined. A lipid emulsion reversed the vasodilation induced by bupivacaine during sodium orthovanadate-induced contraction. The lipid emulsion attenuated the bupivacaine-mediated inhibition of the sodium orthovanadate-induced phosphorylation of protein tyrosine, c-Jun NH2-terminal kinase (JNK), myosin phosphatase target subunit 1 (MYPT1), phospholipase C (PLC) -1 and extracellular signal-regulated kinase (ERK). These results suggest that a lipid emulsion reverses toxic-dose bupivacaine-induced vasodilation during sodium orthovanadate-induced contraction via the activation of a pathway involving either tyrosine kinase, JNK, Rho-kinase and MYPT1 or tyrosine kinase, PLC -1 and ERK, and this reversal is associated with the lipid solubility of the local anesthetic and the induction of calcium sensitization.
provenance
Made available in Cube on 2018-09-28T10:26:11Z (GMT). No. of bitstreams: 0
language
English
author
Ok, Seong-Ho
Lee, Soo Hee
Kwon, Seong-Chun
Choi, Mun Hwan
Shin, Il-Woo
Kang, Sebin
Park, Miyeong
Hong, Jeong-Min
Sohn, Ju-Tae
accessioned
2018-09-28T10:26:11Z
available
2018-09-28T10:26:11Z
issued
2017
citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(18): 2
issn
1422-0067
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473686.do
Funder
교육부
Funding Program
개인기초연구(교육부)
Project ID
1345271905
Jurisdiction
Rep.of Korea
Project Name
Effect of lipid emulsion on the vascular response induced by local anesthetics and cellular mechanism associated with local anesthetic-induced vasoconstriction
rights
openAccess
subject
lipid emulsion
bupivacaine
vasodilation
sodium orthovanadate
tyrosine kinase
myosin phosphatase target subunit
aorta
type
article


Files in This Item

There are no attached files.