A late-lineage murine neutrophil precursor population exhibits dynamicchanges during demand-adapted granulopoiesis

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473681.do
DOI
10.1038/srep39804
Title
A late-lineage murine neutrophil precursor population exhibits dynamicchanges during demand-adapted granulopoiesis
Description
We thank Drs R.M. Locksley, C.A. Lowell (UCSF), I.L. Weissman(Stanford), and G.O. Ahn (Postech) for providing valuable mice, reviewof the manuscript and helpful suggestions, and Drs C. Kang, J.J. Song,M.Y. Kim, D. Lee, and J. Kim (KAIST) and lab members for scientificdiscussions. This work was supported by grants from the NationalResearch Foundation of Korea funded by the Korean Ministry of Science,ICT and future planning [NRF-2011-0020334 and NRF-2012M3A9B4027955 (S-J.Kang), NRF-2012M3A9B4027957 (D. Kim), and NRF-2012M3A9B4027954 (S-Y.Kim)].
abstract
Homeostasis of neutrophils-the blood cells that respond first to infection and tissue injury-is critical for the regulation of immune responses and regulated through granulopoiesis, a multistage process by which neutrophils differentiate from hematopoietic stem cells. Granulopoiesis is a highly dynamic process and altered in certain clinical conditions, such as pathologic and iatrogenic neutropenia, described as demand-adapted granulopoiesis. The regulation of granulopoiesis under stress is not completely understood because studies of granulopoiesis dynamics have been hampered by technical limitations in defining neutrophil precursors. Here, we define a population of neutrophil precursor cells in the bone marrow with unprecedented purity, characterized by the lineage(-)CD11b(+)Ly6G(lo)Ly6B(int)CD115(-), which we call NeuPs (Neutrophil Precursors). We demonstrated that NeuPs differentiate into mature and functional neutrophils both in vitro and in vivo. By analyzing the gene expression profiles of NeuPs, we also identified NeuP stage-specific genes and characterized patterns of gene regulation throughout granulopoiesis. Importantly, we found that NeuPs have the potential to proliferate, but the proliferation decreased in multiple different hematopoietic stress settings, indicating that proliferating NeuPs are poised at a critical step to regulate granulopoiesis. Our findings will facilitate understanding how the hematopoietic system maintains homeostasis and copes with the demands of granulopoiesis.
provenance
Made available in Cube on 2018-09-28T10:26:04Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Min-Hyeok
Yang, Dongchan
Kim, Mirang
Kim, Seon-Young
Kim, Dongsup
Kang, Suk-Jo
accessioned
2018-09-28T10:26:04Z
available
2018-09-28T10:26:04Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473681.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345273975
Jurisdiction
Rep.of Korea
Project Name
BK21 PLUS BioKAIST Initiative
rights
openAccess
type
article


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