A Dalbergia odorifera extract improves the survival of endotoxemia modelmice by inhibiting HMGB1 release

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473548.do
DOI
10.1186/s12906-017-1725-0
Title
A Dalbergia odorifera extract improves the survival of endotoxemia modelmice by inhibiting HMGB1 release
Description
This paper was supported by Konkuk University in 2015.
abstract
Background: Dalbergia odorifera T. Chen (Leguminosae) is an indigenous medicinal herb that is widely used as a popular remedy in northern and eastern Asia. However, the cellular mechanisms underlying the biological activity of D. odorifera are not fully elucidated.
Methods: Anti-inflammatory effect of D. odorifera extract (DOE) was determined through intraperitoneal injection in a mouse model of endotoxemia induced by lipopolysaccharide (LPS). RAW 264.7 cells, a murine macrophage, were also treated with LPS to generate a cellular model of inflammation, and investigated the anti-inflammatory activity and underlying mechanisms of DOE and its constituent isoliquiritigenin.
Results: DOE dose-dependently inhibited LPS-induced release of high mobility group box 1 (HMGB1), a late proinflammatory cytokine, and decreased cytosolic translocation of HMGB1 in RAW264.7 cells. This inhibitory effect of DOE on HMGB1 release was observed in cells treated with DOE before or after LPS treatment, suggesting that DOE is effective for both treatment and prevention. In addition, DOE significantly inhibited LPS-induced formation of nitric oxide (NO) and expression of inducible NO synthase (iNOS) in a dose-dependent manner. These effects of DOE were accompanied by suppression of HMGB1 release triggered by LPS, suggesting a possible mechanism by which DOE modulates HMGB1 release through NO signaling. Isoriquiritigenin, a constituent of DOE, also attenuated LPS-triggered NO formation and HMGB1 release in RAW264.7 cells, indicating that isoriquiritigenin is an indexing molecule for the anti-inflammatory properties of DOE. Furthermore, c-Jun N-terminal kinase, but not extracellular signal-regulated kinase and p38, mediated D OE-dependent inhibition of HMGB1 release and NO/iNOS induction in RAW 264.7 cells exposed to LPS. Notably, administration of DOE ameliorated survival rates in a mouse model of endotoxemia induced by LPS, where decreased level of circulating HMGB1 was observed.
Conclusion:
provenance
Made available in Cube on 2018-09-28T10:22:35Z (GMT). No. of bitstreams: 0
language
English
author
Choi, Hyuk Soo
Park, Jin-A
Hwang, Jung Seok
Ham, Sun Ah
Yoo, Taesik
Lee, Won Jin
Paek, Kyung Shin
Shin, Ho-Chul
Lee, Chi-Ho
Seo, Han Geuk
accessioned
2018-09-28T10:22:35Z
available
2018-09-28T10:22:35Z
issued
2017
citation
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE(17)
issn
1472-6882
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473548.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345274241
Jurisdiction
Rep.of Korea
Project Name
Animal Food Project Team
rights
openAccess
subject
Dalbergia Odorifera
Endotoxemia
HMGB1
Inflammation
Nitric oxide
type
article


Files in This Item

There are no attached files.