A comparison of Ki-67 counting methods in luminal Breast Cancer: TheAverage Method vs. the Hot Spot Method

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473509.do
DOI
10.1371/journal.pone.0172031
Title
A comparison of Ki-67 counting methods in luminal Breast Cancer: TheAverage Method vs. the Hot Spot Method
Description
This study was funded by a grant from the National Research Foundationof Korea (NRF)'s Basic Science Research Program funded by the Ministryof Science, ICT and Future Planning (Grant No. NRF-2015R1A2A2A01007907)to Park SY. The funder had no role in study design, data collection andanalysis, decision to publish, or preparation of the manuscript.
abstract
In spite of the usefulness of the Ki-67 labeling index (LI) as a prognostic and predictive marker in breast cancer, its clinical application remains limited due to variability in its measurement and the absence of a standard method of interpretation. This study was designed to compare the two methods of assessing Ki-67 LI: the average method vs. the hot spot method and thus to determine which method is more appropriate in predicting prognosis of luminal/HER2-negative breast cancers. Ki-67 Lls were calculated by direct counting of three representative areas of 493 luminal/HER2-negative breast cancers using the two methods. We calculated the differences in the Ki-67 Lls (Delta Ki-67) between the two methods and the ratio of the Ki-67 Lls (H/A ratio) of the two methods. In addition, we compared the performance of the Ki-67 Lls obtained by the two methods as prognostic markers. AKi-67 ranged from 0.01% to 33.3% and the H/A ratio ranged from 1.0 to 2.6. Based on the receiver operating characteristic curve method, the predictive powers of the KI-67 LI measured by the two methods were similar (Area under curve: hot spot method, 0.711; average method, 0.700). In multivariate analysis, high Ki-67 LI based on either method was an independent poor prognostic factor, along with high T stage and node metastasis. However, in repeated counts, the hot spot method did not consistently classify tumors into high vs. low Ki-67 LI groups. In conclusion, both the average and hot spot method of evaluating Ki-67 LI have good predictive performances for tumor recurrence in luminal/HER2-negative breast cancers. However, we recommend using the average method for the present because of its greater reproducibility.
provenance
Made available in Cube on 2018-09-28T10:21:31Z (GMT). No. of bitstreams: 0
language
English
author
Jang, Min Hye
Kim, Hyun Jung
Chung, Yul Ri
Lee, Yangkyu
Park, So Yeon
orcid
Park, So Yeon/0000-0002-0299-7268
accessioned
2018-09-28T10:21:31Z
available
2018-09-28T10:21:31Z
issued
2017
citation
PLOS ONE(12): 2
issn
1932-6203
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473509.do
Funder
과학기술정보통신부
Funding Program
개인기초연구(미래부)
Project ID
1711052363
Jurisdiction
Rep.of Korea
Project Name
Evaluation of tumor heterogeneity as a predictive factor for response to treatment and tumor progression
rights
openAccess
type
article


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