A bioinspired dual-crosslinked tough silk protein hydrogel as aprotective biocatalytic matrix for carbon sequestration

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473475.do
DOI
10.1038/am.2017.71
Title
A bioinspired dual-crosslinked tough silk protein hydrogel as aprotective biocatalytic matrix for carbon sequestration
Description
This work was supported by the Energy Efficiency & Resources CoreTechnology Program (20142020200980) of the Korea Institute of EnergyTechnology Evaluation and Planning grant funded by the Ministry ofTrade, Industry and Energy, Korea (to HJ Cha), a Marine BiomaterialsResearch Center grant from the Marine Biotechnology Program of the KoreaInstitute of Marine Science & Technology Promotion funded by theMinistry of Oceans and Fisheries, Korea (to HJ Cha and CS Kim), and theBasic Science Research Program (2016R1D1A3B03933824) of the NationalResearch Foundation funded by the Ministry of Education, Korea (to CSKim).
abstract
The development of carbonic anhydrase (CA)-based materials for the environment-friendly sequestration of carbon dioxide (CO2) under mild conditions would be highly valuable for controlling emissions to the environment and for producing value-added chemicals. Here, a highly tough and stable CA-encapsulating silk protein hydrogel was developed as a robust biocatalyst for CO2 sequestration through a bioinspired dual-crosslinking strategy that employed photoinduced dityrosine chemical crosslinking followed by dehydration-mediated physical crosslinking. The target enzyme was efficiently encapsulated in the silk hydrogel with similar to 60% retention of the activity of free CA, and the encapsulated CA exhibited excellent overall multi-use, storage and thermal stabilities. The dual-crosslinked CA-encapsulating silk hydrogel exhibited a significant compressive modulus, which surpassed the moduli of most traditional and double-network hydrogels as well as those of enzyme-encapsulated hydrogels. This hydrogel also showed high resiliency and elasticity and outstanding structural stability. Importantly, the dual-crosslinked CA-encapsulating silk hydrogel facilitated the sequestration of CO2 into calcium carbonate with high CO2 hydration activity. Thus, the unique combination of bioinspired dual-crosslinking with silk fibroin protein and CA enzyme demonstrates the successful application of this protein hydrogel as a promising biocatalyst for CO2 sequestration by showing high activity, strong mechanical properties and outstanding structural stability.
provenance
Made available in Cube on 2018-09-28T10:20:36Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Chang Sup
Yang, Yun Jung
Bahn, So Yeong
Cha, Hyung Joon
accessioned
2018-09-28T10:20:36Z
available
2018-09-28T10:20:36Z
issued
2017
citation
NPG ASIA MATERIALS(9)
issn
1884-4049
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473475.do
Funder
해양수산부
Funding Program
해양수산생명공학기술개발
Project ID
1525006916
Jurisdiction
Rep.of Korea
Project Name
해양 섬유복합소재 및 바이오플라스틱소재 기술개발
rights
openAccess
type
article


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