A bacterial acetyltransferase triggers immunity in Arabidopsis thalianaindependent of hypersensitive response

Collection with item attached
2017
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473466.do
DOI
10.1038/s41598-017-03704-x
Title
A bacterial acetyltransferase triggers immunity in Arabidopsis thalianaindependent of hypersensitive response
Description
This work was carried out with the support of 'Cooperative ResearchProgram for Agriculture Science & Technology Development (Project No.PJ011913)' Rural Development Administration, Republic of Korea, andBioprotection Centre of Research Excellence, New Zealand. J.J and S.Care the recipients of PhD scholarships from Plant & Food ResearchInstitute and Zespri, respectively, in New Zealand.
abstract
Type-III secreted effectors (T3Es) play critical roles during bacterial pathogenesis in plants. Plant recognition of certain T3Es can trigger defence, often accompanied by macroscopic cell death, termed the hypersensitive response (HR). Economically important species of kiwifruit are susceptible to Pseudomonas syringae pv. actinidiae (Psa), the causal agent of kiwifruit bacterial canker. Although Psa is non-pathogenic in Arabidopsis thaliana, we observed that a T3E, HopZ5 that is unique to a global outbreak clade of Psa, triggers HR and defence in Arabidopsis accession Ct-1. Ws-2 and Col-0 accessions are unable to produce an HR in response to Pseudomonas-delivered HopZ5. While Ws-2 is susceptible to virulent bacterial strain Pseudomonas syringae pv. tomato DC3000 carrying HopZ5, Col-0 is resistant despite the lack of an HR. We show that HopZ5, like other members of the YopJ superfamily of acetyltransferases that it belongs to, autoacetylates lysine residues. Through comparisons to other family members, we identified an acetyltransferase catalytic activity and demonstrate its requirement for triggering defence in Arabidopsis and Nicotiana species. Collectively, data herein indicate that HopZ5 is a plasma membrane-localized acetyltransferase with autoacetylation activity required for avirulence.
provenance
Made available in Cube on 2018-09-28T10:20:20Z (GMT). No. of bitstreams: 0
language
English
author
Jayaraman, Jay
Choi, Sera
Prokchorchik, Maxim
Choi, Du Seok
Spiandore, Amandine
Rikkerink, Erik H.
Templeton, Matthew D.
Segonzac, Cecile
Sohn, Kee Hoon
orcid
Templeton, Matthew/0000-0003-0192-9041; Segonzac,
Cecile/0000-0002-5537-7556; Rikkerink, Erik/0000-0003-4682-5078;
Jayaraman, Jay/0000-0003-4959-9467
accessioned
2018-09-28T10:20:20Z
available
2018-09-28T10:20:20Z
issued
2017
citation
SCIENTIFIC REPORTS(7)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473466.do
Funder
교육부
Funding Program
BK21플러스사업(0.5)
Project ID
1345273980
Jurisdiction
Rep.of Korea
Project Name
Program of Bio-Molecular Function
rights
openAccess
type
article


Files in This Item

There are no attached files.