A "turn-on" fluorescent microbead sensor for detecting nitric oxide

Collection with item attached
2015
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473459.do
DOI
10.2147/IJN.S74924
Title
A "turn-on" fluorescent microbead sensor for detecting nitric oxide
Description
This study was supported by a grant from the Fundamental R&D Program forCore Technology of Materials and the R&D Program for Technology of WorldPremier Materials funded by the Ministry of Trade, Industry and Energy,Republic of Korea.
abstract
Nitric oxide (NO) is a messenger molecule involved in numerous physical and pathological processes in biological systems. Therefore, the development of a highly sensitive material able to detect NO in vivo is a key step in treating cardiovascular and a number of types of cancer-related diseases, as well as neurological dysfunction. Here we describe the development of a fluorescent probe using microbeads to enhance the fluorescence signal. Microbeads are infused with the fluorophore, dansyl-piperazine (Ds-pip), and quenched when the fluorophore is coordinated with a rhodium (Rh)-complex, ie, Rh-2(AcO-)(4)(Ds-pip). In contrast, they are able to fluoresce when the transition-metal complex is replaced by NO. To confirm the "on/off" mechanism for detecting NO, we investigated the structural molecular properties using the Fritz Haber Institute ab initio molecular simulations (FHI-AIMS) package. According to the binding energy calculation, NO molecules bind more strongly and rapidly with the Rh-core of the Rh-complex than with Ds-pip. This suggests that NO can bond strongly with the Rh-core and replace Ds-pip, even though Ds-pip is already near the Rh-core. However, the recovery process takes longer than the quenching process because the recovery process needs to overcome the energy barrier for formation of the transition state complex, ie, NO-(AcO-)(4)-(Ds-pip). Further, we confirm that the Rh-complex with the Ds-pip structure has too small an energy gap to give off visible light from the highest unoccupied molecular orbital/lowest unoccupied molecular orbital energy level.
provenance
Made available in Cube on 2018-09-28T10:20:08Z (GMT). No. of bitstreams: 0
language
English
author
Yang, Lan-Hee
Ahn, Dong June
Koo, Eunhae
accessioned
2018-09-28T10:20:08Z
available
2018-09-28T10:20:08Z
issued
2015
citation
INTERNATIONAL JOURNAL OF NANOMEDICINE(10)
issn
1178-2013
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473459.do
Funder
산업통상자원부
Funding Program
소재부품기술개발
Project ID
1415142723
Jurisdiction
Rep.of Korea
Project Name
Development of PNA-nanohybrid material and cancer diagnostic system with femto-mole sensitivity
rights
openAccess
subject
nitric oxide
microbead
fluorescence
rhodium complex
ab initiomolecular simulation
type
article


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