6-Gingerol reduces Pseudomonas aeruginosa biofilm formation andvirulence via quorum sensing inhibition

Collection with item attached
2015
Item details URL
http://open-repository.kisti.re.kr/cube/handle/open_repository/473454.do
DOI
10.1038/srep08656
Title
6-Gingerol reduces Pseudomonas aeruginosa biofilm formation andvirulence via quorum sensing inhibition
Description
We would like to thank Samantha Reuter for proofreading this manuscript,Kiwon Ok for generating Figure 3 using PyMOL software. This research wasfunded by National Research Foundation of Korea grant(2012-R1A1A11010000 to Y.B. and 2012-R1A1A2043292 to H.-D.P.).
abstract
Pseudomonas aeruginosa is a well-known pathogenic bacterium that forms biofilms and produces virulence factors via quorum sensing (QS). Interfering with normal QS interactions between signal molecules and their cognate receptors is a developing strategy for attenuating its virulence. Here we tested the hypothesis that 6-gingerol, a pungent oil of fresh ginger, reduces biofilm formation and virulence by antagonistically binding to P. aeruginosa QS receptors. In silico studies demonstrated molecular binding occurs between 6-gingerol and the QS receptor LasR through hydrogen bonding and hydrophobic interactions. Experimentally 6-gingerol reduced biofilm formation, several virulence factors (e.g., exoprotease, rhamnolipid, and pyocyanin), and mice mortality. Further transcriptome analyses demonstrated that 6-gingerol successfully repressed QS-induced genes, specifically those related to the production of virulence factors. These results strongly support our hypothesis and offer insight into the molecular mechanism that caused QS gene repression.
provenance
Made available in Cube on 2018-09-28T10:20:00Z (GMT). No. of bitstreams: 0
language
English
author
Kim, Han-Shin
Lee, Sang-Hoon
Byun, Youngjoo
Park, Hee-Deung
accessioned
2018-09-28T10:20:00Z
available
2018-09-28T10:20:00Z
issued
2015
citation
SCIENTIFIC REPORTS(5)
issn
2045-2322
uri
http://open-repository.kisti.re.kr/cube/handle/open_repository/473454.do
Funder
교육부
Funding Program
BK21플러스사업
Project ID
1345244296
Jurisdiction
Rep.of Korea
Project Name
Innovative Leaders for Creating Future Value in Civil Engineering
rights
openAccess
type
article


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